1. Plasminogen domain resembles
A. Fibrinogen
B. LDL receptor
C. Apolipoprotein
D. Prothrombin
Answer: C
2. Boiled cabbage or rancid butter smelling urine is seen in
A. Phenylketonuria
B. Tyrosinemia
C. Isovaleric Acidaemia
D. Multiple carboxylase deficiency
Answer: B
3. True about G protein coupled receptors is:
A. G proteins bind to hormones on the cell surface
B. All the three subunits alpha, beta and gamma should bind to
each other for G protein to act
C. G proteins act as inhibitory and excitatory because of difference
in alpha subunit
D. G protein is bound to GTP in resting state
Answer: C
4. Respiratory quotient of carbohydrate is:
A. 0.5
B. 0.8
C. 0.75
D. 1
Answer: D
5. Essential fatty acids are except:
A. Arachidonic acid
B. Linoleic acid
C. Palmitic acid
D. Linolenic acid
Answer: A
6. The predominant isozyme of LDH in Lung is:
A. LD-1
B. LD-2
C. LD-3
D. LD-5
Answer: C
7. Substrate level phosphorylation occur in step catalysed by which of the following enzyme in TCA cycle?
A. Isocitrate dehydrogenase
B. Malate dehydrogenase
C. Aconitase
D. Succinate thiokinase
Answer: D
8. Phosphofructokinase-1 occupies a key position in regulating glycolysis and is also subjected to feedback control. Which among the following is the allosteric activators of phosphofructokinase-1?
A. Fructose 2, 3 bisphosphate
B. Fructose 2, 6 bisphosphate
C. Glucokinase
D. PEP
Answer: B
9. Enzymes of glycolysis are found in:
A. Cytosol
B. Cell membrane
C. Mitochondria
D. Ribososmes
Answer: A
10. The Fenton reaction leads to free radical generation when:
A. Radiant energy is absorbed by water
B. Ferrous ions are converted to ferric ions
C. Nitric oxide is converted to peroxynitrite anion
D. Hydrogen peroxide is formed by, myeloperoxidase
Answer: B
11. The mechanism of action of uncouplers of oxidative phosphorylation involves:
A. Inhibition of ATP synthase
B. Stimulation of ATP synthase
C. Disruption of proton gradient across the inner membrane
D. Disruption of proton gradient across outer membrane
Answer: C
12. Which of the following is the rate limiting step in cholesterol synthesis?
A. HMG CoA synthase
B. HMG CoA reductase
C. Thiokinase
D. Mevalonate kinase
Answer: B
13. Which one of the following can be a homologous substitution for isoleucine in a protein sequence?
A. Methionine
B. Aspartic acid
C. Valine
D. Arginine
Answer: C
14. Albinism is due to deficiency of the following enzyme?
A. Phenylalanine hydroxylase
B. Homogentisic acid oxidase
C. Tyrosinase
D. Decarboxylase
Answer: C
15. Which among the following glucose transporter present in beta cells ?
A. GLUT1
B. GLUT2
C. GLUT3
D. GLUT4
Answer: B
16. What is the role of catabolite activator protein (CAP) in LAC operon?
A. Positive regulator
B. Negative regulator
C. Attenuation
D. Constitutive expression
Answer: A
17. True about glutamate dehydrogenase is A/E
A. Liver mitochondrial enzyme
B. Use both NAD* or NADP+ coenzyme
C. Inhibited by ADP & activated by GTP
D. Reversible oxidative deamination
Answer: C
18. Among the following, the maximum redox potential is for:
A. NADH/NAD
B. Succinate/Fumarate
C. Ubiquinone
D. Fe+3/ Fe+2
Answer: D
19. (ß-oxidation of palmitic acid yields
A. 3 acetyl CoA
B. 129 ATP net
C. 131 ATP net
D. 16 Acetyl CoA
Answer: B
20. Refsum’s disease is due to deficiency of which of the following enzyme?
A. Malonate dehydrogease
B. Thiophorase
C. Succinate thiokinase
D. Phytanic alpha oxidase
Answer: D
21. Energy source used by brain in later days of Starvation is
A. Glucose
B. Ketone bodies
C. Glycogen
D. Fatty acids
Answer: B
22. In starvation, nitrogen is carried from muscle to liver and kidney by:
A. Alanine
B. Aspartic acid and Serine
C. Glycine
D. Asparagines
Answer: A
23. The gaps between segments of DNA on the lagging strand produced by restriction enzymes are re-joined sealed by:
A. DNA Ligases
B. DNA Helicase
C. DNA topoisomerase
D. DNA phosphorylase
Answer: A
24. Replication and transcription are similar processes in mechanistic terms because both :
A. Use RNA primers for initiation.
B. Use deoxyribonucleotides as precursors.
C. Are semi conserved events
D. Involve phosphodiester bond formation with elongation
occurring in the 5′ – 3′ direction
Answer: D
25.The anticodon region is an important part of the
A. r-RNA
B. m-RNa
C. t-RNa
D. hn-RNa
Answer: C
26. Km value is defined as:
A. Substrate concentration at Vmax/2
B. Substrate concentration of twice Vmax
C. Substrate concentration of thrice Vmax
D. Substrate concentration of one third Vmax
Answer: A
27. Enzyme activity is expressed as:
A. Millimoles /lit?
B. Milli gm/lit?
C. Mg/ dl
D. Micromoles/min
Answer: D
28. Which of the following is a serine protease
A. Chymotrypsin
B. Pepsin
C. Carboxypeptidase
D. Caspases
Answer: A
29. Creatinine is formed from :
A. Arginine
B. Lysine
C. Leucine
D. Histamine
Answer: A
30. Acidic amino acids are
A. Asparagine
B. Arginine
C. None
D. Lysine
Answer: A
31. Non-Essential amino acid is
A. Tyrosine
B. Phenylalanine
C. Lysine
D. Threonine
Answer: A
32. Detergent action of bile salts is due to:
A. Hydropathic
B. Acts as a zwitter ion
C. Amphipathic
D. All
Answer: C
33. Heme synthesis requires
A. Ferrous iron
B. Glycine
C. Succhyl CoA
D. All
Answer: D
34. Tumor suppressor gene is not involved in ?
A. Breast cancers
B. Neurofibromatosis
C. Multiple endocrine neoplasia
D. Retinoblastoma
Answer: C
35. In Endometrial carcinoma, which of the following tumor suppressor gene occurs?
A. P53
B. Rb
C. PTEN
D. APC
Answer: C
36. Telomerase
A. RNA polymerase
B. Causes carcinogenesis
C. Present in somatic cells
D. Absent in germ cells
Answer: B
37. E cadherin gene deficiency is seen in
A. Gastric ca
B. Intestinal ca
C. Thyroid ca
D. Pancreatic ca
Answer: A
38. Li–Fraumeni syndrome is due to mutation of which gene
A. P 21
B. P 53
C. P 41
D. P 43
Answer: B
39. Which element is required by phosphofructokinase?
A. Magnesium
B. Inorganic phosphate
C. Manganese
D. Copper
Answer: A
40. Carbamoyl phosphate synthetase I is:
A. Lysosolic enzyme
B. Cytosolic enzyme
C. Mitochondrial enzyme
D. All of the above
Answer: C
41. Which of the following is required for proper effects of Insulin?
A. Selenium
B. Iron
C. Copper
D. Chromium
Answer: D
42. Glycemic index is defined as:
A. Glucose control in last 3 months
B. Measure of the change in the blood glucose following ingestion of proteins
C. Measure of the change in the blood glucose following ingestion of carbohydrate
D. Measure of the change in the blood glucose following ingestion of fats.
Answer: C
43. Which of the following is known as suicidal enzyme?
A. Lipoxygenase
B. Cyclooxygenase
C. Thromboxane synthatase
D. 5′ nucleotidase
Answer: B
44. Which of the following enzyme is not a component of fatty acid synthase complex?
A. Acetyl Co-A carboxylase
B. Ketoacyl synthase
C. Enoyl reductase
D. Acetoacetyl
Answer: A
45. Umami taste is evoked by ?
A. Glucose
B. Glutamic acid
C. Quinine
D. Sodium chloride
Answer: B
46. Sirtuins are associated with ?
A. Memory
B. Metabolism
C. Vision
D. Olfaction
Answer: B
47. Normal uric acid level is ?
A. 1-2 mg/dl
B. 2-3 mg/dl
C. 3-6 mg/dl
D. 10-15
Answer: C
48. All are true about phosphorus except ?
A. Comprises 1 % of the total body weight
B. 85% remains in the bones
C. Diet is not a common source
D. Parathormone acts on NaPiIIc receptors
Answer: D
49. Carbohydrate in ABO blood group antigens is ?
A. Glucose
B. Fructose
C. Inulin
D. Maltose
Answer: B
50. In argininosuccinase deficiency, what should be supplemented to continue the urea cycle ?
A. Aspartate
B. Arginine
C. Citrullin
D. Argininosuccinate
Answer: B
51. Tay-Sach disease is due to deficiency of
A. Hexosaminidase A
B. Hexosaminidase B
C. Sphingomyelinase
D. a-galactosidase
Answer: A
52. Maximum energy is liberated by hydrolysis of ?
A. Creatine phosphate
B. ATP
C. Phosphoenol pyruvate
D. Glucose-6-phosphate
Answer: C
53. Which of the following is an aldose sugar?
A. Ribulose
B. Fructose
C. Glyceraldehyde
D. All of the above
Answer: C
54. Bond involved in formation of primary structure of protein/polypeptide ?
A. Hydrogen
B. Peptide
C. Disulfide
D. a and b both
Answer: D
55. Main source of energy in 1 min is ?
A. Glycogen
B. FFA
C. Phosphates
D. Glucose
Answer: A
56. Quarternary ammonium compound disinfectants are ?
A. Anionic
B. Cationic
C. Neutral
D. Gases
Answer: B
57. Not a component of PCR ?
A. Primer
B. Taq polymerase
C. DNA Polymerase
D. Restriction enzyme
Answer: D
58. Which of the following is increased in lipoprotein lipase deficiency?
A. VLDL
B. LDL
C. HDL
D. Chylomicrons
Answer: D
59. NADH CoQ reductase is inhibited by ?
A. Rotenone
B. Carbonmonoxide
C. Antimycin
D. Atractyloside
Answer: A
60. Rate limiting step in urea cycle is catalyzed by ?
A. Arginase
B. Argininosuccinase
C. Carbamoyl-phosphate synthase
D. Ornithine transcarbamylase
Answer: C
61. Citrullinemia is due to deficiency of ?
A. Argininosuccinate lyase
B. Argininosuccinate synthase
C. Arginase
D. Ornithine transcarbamylase
Answer: B
62. Urease is a/an ?
A. Oxidoreductase
B. Lyase
C. Ligase
D. Hydrolase
Answer: D
63. Vitamin involved in decarboxylation ?
A. Biotin
B. Pyridoxine
C. Niacin
D. Thiamine
Answer: B
64. Nitrogen-9 of purine ring is provided by ?
A. Glycine
B. Aspartate
C. Glutamine
D. CO2
Answer: C
65. Rate limiting step in fatty acid synthesis is ?
A. Production of acetyl CoA
B. Production of oxaloacetate
C. Production of malonyl-CoA
D. Production of citrate
Answer: C
66. Which is not a common enzyme for glycolysis and gluconeogenesis?
A. Aldolase
B. Glucose-6-phosphatase
C. Phosphoglycerate mutase
D. Phosphoglycerate kinase
Answer: B
67. Which is not a step of gluconeogenesis?
A. Conversion of glucose-6-phosphate to glucose
B. Carboxylation of pyruvate
C. Conversion of oxaloacetate to phosphoenolpyruvate
D. Conversion of phosphoenolpyruvate to pyruvate
Answer: D
68. Vitamin C is required for ?
A. Posttranslational modification
B. Synthesis of epinephrine
C. Tyrosine metabolism
D. All of the above
Answer: D
69. First product of purine metabolism
A. Uric acid
B. Xanthine
C. P-alanine
D. CO2
Answer: B
70. Which of the following metabolic pathway in carbohydrate metabolism is required for synthesis of nucleic acids?
A. Gluconeogenesis
B. Glycolysis
C. HMP shunt
D. Glycogenesis
Answer: C
71. Mineral required for cholesterol biosynthesis ?
A. Fe
B. Mn
C. Mg
D. Cu
Answer: C
72. Source of energy in Kreb’s cycle is
A. NAD
B. NADP
C. NADPH
D. NADH
Answer: D
73. Coenzyme used in Kreb’s cycle ?
A. NAD
B. NADP
C. NADPH
D. NADH
Answer: A
74. Mousy odor of urine is seen in ?
A. Alkaptunuria
B. Phenylketonuria
C. Hartnup disease
D. Albinism
Answer: B
75. Cabbage-like odour is seen in ?
A. Alkaptonuria
B. Phenylketonuria
C. Hartnup disease
D. Tyrosinemia
Answer: D
76. Immediate energy supply for muscle contraction ?
A. GTP
B. ATP
C. Creatine phosphate
D. Fatty acid
Answer: C
77. Cofactor for dopamine hydroxylase ?
A. Fe
B. Mg
C. Mn
D. Cu
Answer: D
78. Lipoprotein involved in reverse cholesterol transport?
A. LDL
B. VLDL
C. IDL
D. HDL
Answer: D
79. Allantoin is the end product of metabolism of ?
A. Glycogen
B. Purine
C. Pyrimidine
D. Histidine
Answer: B
80. Pyruvate dehydrogenase contains all, except
A. NAD
B. FAD
C. Biotin
D. CoA
Answer: C
81. Northern blot is used to detect ?
A. Protein
B. Immunoglobulin
C. RNA
D. DNA
Answer: C
82. Not an essential amino acid ?
A. Arginine
B. Histidine
C. Glutamate
D. Lysine
Answer: C
83. Enzyme deficient in gangliosidoses ?
A. ß-glucuronidase
B. Iduronidase
C. ß-galactosidase
D. Hyaluronidase
Answer: C
84. Defect in type II hyperlipidemia
A. Apo-E
B. Lipoprotein lipase
C. LDL receptor
D. None
Answer: C
85. Major source of energy for brain in fasting/ starvation ?
A. Glucose
B. Glycogen
C. Fatty acids
D. Ketone bodies
Answer: D
86. Nicotinic acid is derived from ?
A. Glutamine
B. Tryptophan
C. Glutathione
D. Phenylalanine
Answer: B
87. Sex determining region is located on ?
A. Long arm of Y chromosome
B. Short arm of Y chromosome
C. Long arm of X chromosome
D. Short arm of X chromosome
Answer: B
88. Transferases are classified as ?
A. EC-1
B. EC-2
C. EC-3
D. EC-4
Answer: B
89. True about acid phosphatase is ?
A. Acts at pH 8-9
B. Prostate isoform is tartarate resistant
C. Erythrocyte isoform is inhibited by cupric ions
D. All of the above
Answer: C
90. Alcohol is metabolized by ?
A. Alcohol dehydrogenase
B. MEOS
C. Catalase
D. All of the above
Answer: D
91. Amino acid which is optically inert ?
A. Valine
B. Alanine
C. Glycine
D. Threonine
Answer: C
92. First purine nucleotide, which is synthesized in purine biosynthesis ?
A. AMP
B. GMP
C. IMP
D. UMP
Answer: C
93. Rate limiting step in heme synthesis is catalyzed by ?
A. ALA dehydratase
B. ALA synthase
C. UPG decarboxylase
D. Ferrochelatase
Answer: B
94. Rate limiting step in porphyrine synthesis
A. ALA dehydratase
B. ALA synthase
C. UPG decarboxylase
D. Ferrochelatase
Answer: B
95. Oxidation of very long chain fatty acids takes place in ?
A. Cytosol
B. Mitochondria
C. Ribosomes
D. Peroxisomes
Answer: D
96. Salvage pathway of purine biosynthesis is important for ?
A. Liver
B. RBCs
C. Kidney
D. Lung
Answer: B
97. Lipoprotein associated with carrying cholesterol from peripheral tissues to liver is ?
A. HDL
B. LDL
C. VLDL
D. IDL
Answer: A
98. Which of the following is a nucleoside?
A. Adenine
B. Uridine
C. Thymine
D. Guanine
Answer: B
99. Amino acids with extra NH (amino group) in structure2
A. Aspartate
B. Glutamate
C. Histidine
D. Alanine
Answer: C
100. Number of ATP produced by RBC when Glycolysis occurs through Rapoport Leubering pathway
A. 2
B. 6
C. 8
D. 0
Answer: D
101. Most important vitamin, which promotes wound healing ?
A. Vitamin C
B. Vitamin D
C. Vitamin A
D. Niacin
Answer: A
102. Which of the following is not a glycerosphingolipid?
A. Lecithin
B. Cardiolipin
C. Plasmalogens
D. Sphingomyelin
Answer: D
103. Immediate precursor of creatine
A. Carbamoyl phosphate
B. Arginosuccinate
C. Guanidoacetate
D. Citrulline
Answer: C
104. Tyrosine enters gluconeogenesis by forming which substrate
A. Succinyl CoA
B. Alpha-ketoglutarate
C. Fumarate
D. Citrate
Answer: C
105. Fumarate of TCA is derived from transmination of which amino acid
A. Phenylalanine
B. Methionine
C. Valine
D. Glutamine
Answer: A
106. Alpha helix and Beta pleated sheet are examples of?
A. Primary
B. Secondary structure
C. Tertiary
D. Quaternary structure
Answer: B
107. RNA polymerase has which activity
A. Primase
B. Helicase
C. Ligase
D. Topoisomerase
Answer: A
108. which of the following occurs only in mitochondria
A. ECT
B. Ketogenesis
C. Urea cycle
D. Steroid synthesis
Answer: C
109. Number of structural gene in Lac operon
A. 3
B. 4
C. 5
D. 6
Answer: A
110. The enzyme involved in initiation of peptide chain synthesis
A. Topoisomerase
B. Transformylase
C. RNA polymerase
D. Peptidyl transferase
Answer: B
111. Strongest interactions among the following
A. Covalent
B. Hydrogen
C. Electrostatic
D. Van der Waals
Answer: A
112. Highest mobility on electrophoresis
A. HDL
B. VLDL
C. LDL
D. Chylomicrons
Answer: A
113. Regulating enzymes in Gluconeogenesis are all, except
A. Pyruvate carboxylase
B. PEP carboxykinase
C. PFK-1
D. Glucose-6-phosphatase
Answer: C
114. Glucagon stimulates
A. Gluconeogenesis
B. Glycogenesis
C. Fatty acid synthesis
D. Glycolysis
Answer: A
115. Which of the following amino acids is purely ketogenic?
A. Phenylalanine
B. Leucine
C. Proline
D. Tyrosine
Answer: B
116. Cystathionine lyase requires which cofactor ?
A. Thiamine
B. Riboflavin
C. Pyridoxine
D. Niacin
Answer: C
117. Most common physiological form of DNA
A. A-form
B. B-form
C. Z-form
D. C-form
Answer: B
118. CO acts by inhibiting which component of respiratory chain ?
A. Cytochrome b
B. Cytochrome C oxidase
C. NADH CoQ reductase
D. Oxidative phosphorylation
Answer: B
119. Specific inhibitor of succinate dehydrogenase?
A. Fluoroacetate
B. Arsenite
C. Malonate
D. Fluoride
Answer: C
120. In citric acid cycle, NADH is produced by
A. Succinate thiokinase
B. Succinate dehydrogenase
C. Isocitrate dehydrogenase
D. Fumarase
Answer: C
121. Urea is synthesized in all except
A. Liver
B. Brain
C. Kidney
D. Spleen
Answer: D
122. Which of the following step is specific for gluconeogenesis
A. Pyrovate to acetyl CoA
B. Oxaloacetate to citrate
C. Oxaloacetate to PEP
D. Oxaloacetate to PEP
Answer: C
123. Pyruvate can be a substrate for
A. Fatty acid synthesis
B. TCA cycle
C. Cholesterol synthesis
D. All of the above
Answer: D
124. In oxidative pathway, NADPH is produced in ?
A. Cytosol
B. Mitochondria
C. Ribosome
D. Peroxisomes
Answer: A
125. Glucose is converted to sorbitol by ?
A. Aldolase B
B. Aldose reductase
C. Sorbitol dehydrogenase
D. All of these
Answer: B
126. Lactose intolerance is due to ?
A. Deficiency of Galactokinase
B. Deficiency of Uridyl transferase
C. Deficiency of Lactase
D. Deficiency of Enteropeptidase
Answer: C
127. Deficiency of which vitamin causes excretion of xantheurenic acid in urine ?
A. Folic acid
B. Pyridoxin
C. Niacin
D. Vitamin B12
Answer: B
128. Total number of dehydrogenases Krebs cycle ?
A. 3
B. 2
C. 4
D. 5
Answer: C
129. Number of ATP generated in one TCA cycle ?
A. 2
B. 8
C. 10
D. 11
Answer: C
130. If urine sample darkens on standing: the most likely conditions is ?
A. Phenylketonuria
B. Alkaptonuria
C. Maple syrup disease
D. Tyrosinemia
Answer: B
131. Which of the following enzyme does not catalyzes irreversible step in glycolysis ?
A. Hexokinase
B. Phosphoglycerate kinase
C. Pyruvate kinase
D. Phosphofructokinase
Answer: B
132. Taurine is made from ?
A. Glycine
B. Tyrosine
C. Cysteine
D. Phenylalanine
Answer: C
133. Amino acid carrying ammonia from muscle to liver?
A. Alanine
B. Glutamine
C. Arginine
D. Lysine
Answer: A
134. FIGLU excretion test is used for assessment of deficiency of ?
A. Vitamin B12
B. Niacin
C. Folic acid
D. Pyridoxin
Answer: C
135. Role of molecular oxygen in ETC ?
A. Transfer of reducing equivalent to CoQ
B. Transfer of reducing equivalent from cytosol to mitochondria
C. To act as last electron acceptor
D. Generation of ATP
Answer: C
136. Which of the following is a homopolysaccharide?
A. Heparin
B. Chitin
C. Hyaluronic acid
D. Chondroitin sulfate
Answer: B
137. If tyrosine level in blood is normal without external supplementation, deficiency of which of the following is ruled out ?
A. Tryptophan
B. Phenylalanine
C. Histidine
D. Isoleucine
Answer: B
138. End product of porphyrin metabolism ?
A. Albumin
B. CO2 & NH2
C. Bilirubin
D. None
Answer: C
139. Hunter syndrome is due to deficiency of
A. Beta galactosidase
B. Sphingomyelinase
C. Iduronate Sulfatase
D. Hyaluronidase
Answer: C
140. UDP glucose is not used in ?
A. Uronic acid pathway
B. Glycogen synthesis
C. Galactose metabolism
D. HMP shunt
Answer: D
141. Number of ATP molecules and NADH formed in each cycle of glycolysis ?
A. 4 ATP, 2 NADH
B. 2 ATP, 2 NADH
C. 4 ATP, 4 NADH
D. 2 ATP, 4 NADH
Answer: A
142. Which of the following is not true regarding ETC?
A. Occurs in mitochondria
B. Generates ATP
C. No role of inorganic phosphate
D. Involves transport of reducing equivalent
Answer: C
143. Proteins are separated on the basis of charge in ?
A. SDS-PAGE
B. Ultracentrifugation
C. Affinity chromatography
D. HPLC
Answer: D
144. Isoform of LDH in skeletal muscles ?
A. LDH-1
B. LDH-2
C. LDH-3
D. LDH-4
Answer: D
145. Following is true regarding sulhydryl groups except?
A. They are present in coenzyme A and lipoic acid
B. They are present in Captopril and penicillamine
C. They are not involved in reduction of peroxides
D. They are present in cysteine
Answer: C
146. Cause of Herpes Zoster ?
A. Primary infection with VZV
B. Recurrent infection with VZV
C. Reactivation of latent infection of VZV
D. Multiple infection with VZV
Answer: C
147. Bile acids are synthesized from ?
A. Heme
B. Cholesterol
C. Ribulose
D. Arachidonic acid
Answer: B
148. Not a substrate for gluconeogenesis A. Glycerol
B. Leucine
C. Lactate
D. Propionate
Answer: B
149. Rate limiting enzyme in bile acid synthesis ?
A. Desmolase
B. 21 a-hydroxylase
C. 7a-hydroxylase
D. 12a-hydroxylase
Answer: C
150. Rate limiting enzyme in catecholamine synthesis?
A. Dopa decarboxylase
B. N-methyltransferase
C. Dopamine hydroxylase
D. Tyrosine hydroxylase
Answer: D
151. NAD+ Acts as a coenzyme for ?
A. Xanthine oxidase
B. L-amino acid oxidase
C. Succinate dehydrogenase
D. Malate dehydrogenase
Answer: D
152. True about tRNA ?
A. 80% of total RNA
B. Contains 50-60 nucleotides
C. CCA sequence is transcribed
D. Longest RNA
Answer: C
153. Selenocysteine is associated with ?
A. Carbonic anhydrase
B. Catalase
C. Deiodinase
D. Transferase
Answer: C
154. Which vitamin is required for transfer of 1-carbon unit?
A. Vitamin A
B. Folic acid
C. Vitamin B12
D. Niacin
Answer: B
155. Heme is synthesized from ?
A. Lysine + succinyl CoA
B. Glycine + succinyl CoA
C. Arginine + Malonyl CoA
D. Glycine + Malonyl CoA
Answer: B
156. Splicing is a process of ?
A. Activation of protein
B. Removal of introns
C. Synthesis of protein
D. Replication of DNA
Answer: B
157. Creatinine is formed from A. Glycine
B. Lysine
C. Leucine
D. Histamine
Answer: A
158. Most commonly used vector for DNA cloning ?
A. Plasmid
B. Virus
C. Cosmid
D. Phage
Answer: A
159. Richest source of vitamin B12 ?
A. Meat
B. Green leafy vegetables
C. Corn oil
D. Sunflower oil
Answer: A
160. Keshan disease in due to deficiency of
A. Selenium
B. Copper
C. Zinc
D. Iron
Answer: A
161. PFK-I inhibitor ?
A. AMP
B. Citrate
C. Glucose 6 phosphate
D. Insulin
Answer: B
162. All are used in gluconeogenesis except ?
A. Oleate
B. Succinate
C. Glutamate
D. Aspartate
Answer: A
163. Main enzyme for glycogen metabolism ?
A. Glucose-6-phosphatase
B. Glycogen synthase
C. PFK – 1
D. None of the above
Answer: B
164. Action of metalloproteinase ?
A. Degradation of collagen
B. Polymerization of collagen
C. Oxidation of collagen
D. Stimulation of collagen
Answer: A
165. Protein catabolism is increased in ?
A. Starvation
B. Burns
C. Surgery
D. All of the above
Answer: D
166. TATA box is seen in ?
A. Promoter region
B. Palindromic region
C. Enhancer region
D. Silencer region
Answer: A
167. Frame shift mutation does not effect complete amino acid sequence if it occurs in multiple of ?
A. I
B. 2
C. 3
D. None
Answer: C
168. Coris disease is due to defect in
A. Branching enzyme
B. Debranching enzyme
C. Myophosphorylase
D. Hepatic phosphorylase
Answer: B
169. Oxidative deamination is catalyzed by ?
A. Glutaminase
B. Glutamine synthase
C. Glutamate dehydrogenase
D. None of the above
Answer: C
170. Glucose is transported in pancreas through which receptor ?
A. GLUT 1
B. GLUT 2
C. GLUT 3
D. GLUT 4
Answer: B
171. Immediate source of energy is ?
A. Cori’s cycle
B. HMP
C. ATP
D. TCA cycle
Answer: C
172. Which of the following vitamins does not participate in oxidative decarboxylation of pyruvate to acetyl CoA ?
A. Thiamine
B. Niacine
C. Riboflavin
D. Biotin
Answer: D
173. Salvage pathway of purine nucleotide synthesis are used by all except ?
A. Brain
B. Liver
C. RBC
D. Leukocytes
Answer: B
174. Carboxypeptidase contains which mineral ?
A. Copper
B. Zinc
C. Iron
D. None
Answer: B
175. Krabbes disease is due to deficiency of ?
A. Sphingomyelinase
B. Beta galactocerebrosidase
C. Hexosaminidase
D. Arylsulfatase
Answer: B
176. True about gluconeogenesis ?
A. Occurs mainly in muscle
B. It is reverse of glycolysis
C. Alanine & lactate both can serve as substrate
D. Glycerol is not a substrate
Answer: C
177. Which enzyme polymerises okazaki fragments ?
A. DNA polymerase I
B. DNA polymerase II
C. DNA polymerase III
D. RNA polymerase
Answer: C
178. Riboflavin deficiency is assessed by ?
A. Transketolase
B. Glutathione reductase
C. PDH
D. None
Answer: B
179. Heme is which porphyrin ?
A. Type I
B. Type II
C. Type III
D. Type IV
Answer: C
180. Which of the following is a lyase ?
A. Decarboxylase
B. Synthetase
C. Kinase
D. Oxygenase
Answer: A
181. Not present in DNA ?
A. Uracil
B. Thymine
C. Cytosine
D. Adenine
Answer: A
182. Neutral amino acid is ?
A. Aspartate
B. Arginine
C. Glycine
D. Histidine
Answer: C
183. All are true about ketone bodies except ?
A. Acetoacetate is primary ketone body
B. Synthesized in mitochondria
C. Synthesized in liver
D. HMG CoA reductase is the rate-limiting enzyme
Answer: D
184. The mineral having action like vitamin E ?
A. Calcium
B. Iron
C. Selenium
D. Magnesium
Answer: C
185. Which is an abnormal lipoprotein ?
A. VLDL
B. Chylomicron
C. Lp (A.
D. LDL
Answer: C
186. Okazaki fragments are formed during ?
A. Transcription
B. Translation
C. DNA replication
D. None
Answer: C
187. Hay’s sulpher test is used to detect
which of the following ?
A. Bile salts in urine
B. Reducing sugar in urine
C. Ketone bodies in urine
D. Urobilinogen in urine
Answer: A
188. Which of the following accumulates in maple syrup urine disease
A. Leucine
B. Valine
C. Isoleucine
D. All
Answer: D
189. Keratin is a ?
A. Globular protein
B. Cylindrical protein
C. Fibrous protein
D. None of the above
Answer: C
190. Keratin contains ?
A. Arginine
B. Histidine
C. Lysine
D. All
Answer: D
191. Which of the following is high energy compound?
A. ADP
B. Glucose-6-phosphate
C. Creatine phosphate
D. Fructose-6-phosphate
Answer: C
192. True statement about hemoglobin is ?
A. Each hemoglobin molecule is made of 4 polypep tides of each subunit
B. Two alpha and two beta subunits having a 02 attached to each subunit
C. Each hemoglobin molecule binds to only one 02 molecule
D. Each hemoglobin has one heme molecule
Answer: A
193. All are true about LDL except ?
A. More dense than chylomicron
B. Smaller than VLDL
C. Transports maximum amount of lipid
D. Contains maximum cholesterol
Answer: C
194. Gluconeogenesis from lactate needs all except ?
A. Transport of lactate from muscle to liver
B. Conversion of lactate to pyruvate
C. Transamination of pyruvate to alanine
D. None of the above
Answer: C
195. All are true about Vitamin B12,except ?
A. Active form is methylcobalamine
B. Requires for conversion of homocysteine to methionine
C. Requires in metabolism of methylmalonyl CoA
D. Requires for conversion of pyruvate to lactate
Answer: D
196. Polar amino acids are all except ?
A. Glutamic acid
B. Histidine
C. Glutamine
D. Methionine
Answer: D
197. Major form of folic acid to transfer one carbon is ?
A. Methylene THF
B. Formyl THF
C. Methyl THF
D. All
Answer: A
198. During starvation, muscle uses ?
A. Fatty acids
B. Ketone bodies
C. Glucose
D. Proteins
Answer: A
199. Serotonin is derived from A. Tyrosine
B. Tryptophan
C. Phenylalanine
D. Methionine
Answer: B
200. Major apolipoprotein of chylomicrons ?
A. B-100
B. D
C. B-48
D. None
Answer: C
201. True about Glutathione except ?
A. Tripeptide
B. Formed from glutamic acid, glycine, cysteine
C. Act as antioxidant in reduced state
D. All of the above
Answer: D
202. Concentration of which is inversely related to the risk of coronary heart disease ?
A. VLDL
B. LDL
C. HDL
D. None
Answer: C
203. Maximum cholesterol is seen in ?
A. VLDL
B. LDL
C. HDL
D. Chylomicrons
Answer: B
204. In humans, end product of purine metabolism
A. Allantoin
B. Uric acid
C. CO2
D. None
Answer: B
205. All are true about chaperones except ?
A. Cause folding of proteins
B. Are lipid in nature
C. May have ATPase activity
D. Include heat shock proteins
Answer: B
206. Which Vitamin is involved in Redox reactions
A. Pyridoxin
B. Biotin
C. Folic acid
D. Riboflavin
Answer: D
207. Ninhydrin test is used for ?
A. Bile salts
B. Amino acids
C. Nucleic acid
D. Lipids
Answer: B
208. Which is not a dietary fiber ?
A. Lignin
B. Lactulose
C. Pectin
D. Cellulose
Answer: B
209. ATP is generated in ETC by ?
A. Na’ ATPase
B. Cl- ATPase
C. FoF, ATPase
D. ADP Kinase
Answer: C
210. Atractiloside act as ?
A. Uncoupler
B. Inhibitor of oxidative phosphorylation
C. Inhibitor of complex I of ETC
D. Inhibitor of complex III of ETC
Answer: B
211. Riboflavin is a constituent of ?
A. FMN
B. NAD
C. PLP
D. THF
Answer: A
.
212. Rate limiting step in pyrimidine synthesis ?
A. Dihydro-orotase
B. Ornithine transcarbomoylase
C. Aspartate transcarbomoylase
D. Carbamoyl phosphate synthase-I
Answer: C
213. All are characteristics of genetic code ?
A. Overlapping
B. Nonambiguous
C. Universal
D. Degeneracy
Answer: A
214. Which of the following is an example of Trinucleotide repeat mutation ?
A. Huntington’s chorea
B. Fragile-X-syndrome
C. Friedreich ataxia
D. All of the above
Answer: D
215. Which of the following is the major proteoglycan of synovial fluid ?
A. Chondroitin sulfate
B. Dermatan sulfate
C. Heparan sulfate
D. Hyaluronic acid
Answer: D
216. The main function of Vitamin C in the body is
A. Coenzyme for energy metabolism
B. Regulation of lipid synthesis
C. Involvement as antioxidant
D. Inhibition of cell growth
Answer: C
217. Enzyme specificity is given by ?
A. Km
B. Vrm„
C. Both
D. None
Answer: A
218. Natural uncoupler is ?
A. Thermogonin
B. 2, 4 nitrophenol
C. 2, 4 Dinitrophenol
D. Oligomycin
Answer: A
219. Lipid with highest mobility is ?
A. HDL
B. LDL
C. VLDL
D. Chylomicrons
Answer: A
220. Apolipoprotein E is rich in ?
A. Lysine
B. Arginine
C. Histidine
D. Methionine
Answer: B
221. Which energy molecule gives 10.5 kcal/ molecule?
A. ATP
B. GTP
C. Creatine phosphate
D. Glucose-6-phosphate
Answer: C
222. Branching enzyme is found in ?
A. Glycogenesis
B. Glucogenesis
C. Glycogenolysis
D. Glycolysis
Answer: A
223. Which of the following is not a phospholipid ?
A. Lecithine
B. Plasmalogen
C. Cardiolipin
D. Ganglioside
Answer: D
224. The mechanism by which mercury causes damage ?
A. Binds to -SH group of enzyme
B. Directly toxic
C. Inhibits ETC
D. Inhibits protein synthesis
Answer: A
225. Q10 in enzyme matches with ?
A. 2
B. 4
C. 8
D. 10
Answer: A
226. Creatine is made up of all, except ?
A. Glycine
B. Alanine
C. Methionine
D. Arginine
Answer: B
227. In urea cycle which defect is an X linkeddisease ?
A. Ornithine transcarbamylase
B. Aspartate transcarbamylase
C. Arginase
D. Argininosuccinate synthase
Answer: A
228. True regarding collagen synthesis is all except ?
A. Synthesized in ribosomes as preprocollagen
B. Hydroxylation of proline occurs in Golgi apparatus
C. Hydroxylation of lysine occurs in ER
D. Triple helix assembly occurs in ER
Answer: B
229. Type of collagen found in space of Disse in liver is A. Collagen I & II
B. Collagen III & IV
C. Collagen II &
D. Collagen II & V
Answer: B
230. Which is predominant in normal healthy human ?
A. LDH 1
B. LDH2
C. LDH 3
D. LDH4
Answer: B
231. Physiological uncoupler is ?
A. Thyroxine
B. Free fatty acids
C. Thermogenin
D. All of the above
Answer: D
232. Which of the following is Aldosugar ?
A. Fructose
B. Erythrulose
C. Glucose
D. None
Answer: C
233. Key enzyme of gluconeogenesis are all except?
A. Pyruvate carboxylase
B. PEP carboxykinase
C. Pyruvate kinase
D. Glucose-6-phosphatase
Answer: C
234. Fructose intolerance is due to deficiency of ?
A. Aldolase B
B. Fructokinas
C. Triokinas
D. Aldolase A
Answer: A
235. What is essential for transfer of fatty acid across mitochondrial membrane
A. Creatine
B. Creatinine
C. Carnitine
D. None
Answer: C
236. Hexokinase is ?
A. Ligase
B. Transferase
C. Oxidoreductase
D. Reductase
Answer: B
237. Ketone body formation without glycosuria is seen in ?
A. Diabetes mellitus
B. Diabetes insipidus
C. Starvation
D. Obesity
Answer: C
238. Amino acid with double chiral is ?
A. Phenyalanine
B. Threonine
C. Tryptophane
D. Tyrosine
Answer: B
239. At physiological pH DNA is ?
A. Acidic
B. Negatively charged
C. Amphipathic
D. All of the above
Answer: D
240. Source of ammonia in urine ?
A. Glutaminase
B. Urease
C. Glutamate dehydrogenase
D. Arginase
Answer: A
241. Albumin binds with all except ?
A. Steroid
B. Calcium
C. FFA
D. Thyroxine
Answer: D
242. Chemical process involved in conversion of progesterone to glucocorticoids is
A. Methylation
B. Hydroxylation
C. Carboxylation
D. None
Answer: B
243. Gluconeogenesis occurs in all except ?
A. Liver
B. Kidney
C. Gut
D. Muscle
Answer: D
244. GLUT 2 receptors ?
A. Insulin dependent
B. Insulin independent
C. Found in cardiac muscle
D. Found in brain
Answer: B
245. Which of the following has antioxidant property?
A. Selenium
B. Copper
C. Zinc
D. All
Answer: D
246. The number of ATPs generated in krebs cycleare ?
A. 12
B. 24
C. 15
D. 30
Answer: B
247. One Krebs cycle generates how many ATP ?
A. 6
B. 12
C. 24
D. 36
Answer: B
248. Glutathione requires which vitamin to act as antioxidant ?
A. Vitamin E
B. Niacin
C. Vitamin C
D. Vitamin A
Answer: B
249. Metabolic change seen in starvation are all except?
A. Increased gluconeogenesis
B. Increased glycolysis
C. Ketogenesis
D. Protien degradation
Answer: B
250. Catecholamines are synthesized from ?
A. Tryptophan
B. Tyrosine
C. Methionine
D. Histidine
Answer: B
251. Which of the enzyme of glycolysis is a part of gluconeogenesis ?
A. Pyruvate kinase
B. PFK
C. Hexokinase
D. Phosphoglycerate kinase
Answer: D
252. Molecular interaction, found in the structure of DNA
A. Hydrogen bond
B. Glycosidic bond
C. Covalent interactions
D. All of the above
Answer: D
253. Pyridoxine is used in treatment of ?
A. Galactosemia
B. Phenylketonuria
C. Propionic acidemia
D. Homocystinuria
Answer: D
254. Membrane proteins are synthesized in ?
A. Free ribosome
B. Bound ribosome
C. Nucleolus
D. Mitochondria
Answer: B
255. Transmination of Aspartate forms ?
A. Pyruvate
B. Acetly-CoA
C. Oxaloacetate
D. Alanine
Answer: C
256. Interaction involved in primary structure of protein ?
A. Hydrogen bond
B. Disulfide bond
C. Peptide bond
D. Electrostatic bond
Answer: C
257. All are true about vitamin E except ?
A. Act as antioxidant
B. Prevent lipid peroxidation of cell membrane
C. Water soluble vitamin
D. Chemically tocopheral
Answer: C
258. Blood form of folic acid is
A. Folinic acid
B. Pteroglutamate
C. Methyl THE
D. None
Answer: C
259. Primary hypercholesterolemia is ?
A. Type I
B. Type Ha
C. Type IIb
D. Type III
Answer: B
260. Salvage purine synthesis refers to ?
A. Synthesis of purine from ribose-5-phosphate
B. Synthesis of purine from pyrimidine
C. Synthesis of purine nucleotides from purine bases
D. None of the above
Answer: C
261. RNA which contains codon for specific amino acid ?
A. tRNA
B. rRNA
C. mRNA
D. None
Answer: C
262. Glycine is required in formation of all except ?
A. Heme
B. Purines
C. Glutathione
D. Thyroxine
Answer: D
263. True about glycolysis are all except ?
A. Provide nutrition to cancer cells
B. Substrate level phosphorylation at pyruvate kinase
C. Two carbon end product is formed
D. NADPH is formed by glyceraldhyde-3-phosphate dehydrogenase
Answer: C
264. Instant energy to muscle is provided by which pathway?
A. HMP shunt
B. Embden mayerhoff pathway
C. Cori cycle
D. TCA cycle
Answer: B
265. Which steroid is formed from cholesterol without hydroxylation ?
A. Progesterone
B. Glucocorticoid
C. Mineralocorticoid
D. Estradiol
Answer: A
266. Not obtained from plant source ?
A. Cobalamine
B. Riboflavin
C. Thiamine
D. Vitamin A
Answer: A
267. Pseudouridine found in?
A. DNA
B. rRNA
C. mRNA
D. tRNA
Answer: D
.
268. Fatty acids used by all except ?
A. Liver
B. Muscle
C. Brain
D. Kidney
Answer: C
269. Vitamin not required in TCA cycle ?
A. Niacin
B. Riboflavin
C. Thiamine
D. Folic acid
Answer: D
270. Uric acid is formed by ?
A. Catabolism of proteins
B. Catabolism of ketones
C. Catabolism of purines
D. Catabolism fo pyrimidines
Answer: C
271. Anaerobic glycolysis occurs in all places except
A. Muscles
B. RBCs
C. Brain
D. Kidney
Answer: C
272. Sweaty feet odor in urine is seen in ?
A. Phenylketonuria
B. Maple syrup urine
C. Isovaleric acidemia
D. Alkaptonuria
Answer: C
273. Ketone bodies are not used by ?
A. Muscle
B. Brain
C. RBC
D. Renal cortex
Answer: C
274. Which collagen produces sheets ?
A. I
B. II
C. IV
D. VI
Answer: C
275. The energy for glycogenesis is provided by
A. GTP
B. GDP
C. UTP
D. AMP
Answer: C
276. The function of vitamin K largely depends on which mineral ?
A. Selenium
B. Calcium
C. Iron
D. Magnesium
Answer: B
277. Amino acid with aliphatic side chain is?
A. Serine
B. Leucine
C. Threonine
D. Aspartate
Answer: B
278. According to IUB system, hydrolases belong to which class ?
A. EC-1
B. EC-2
C. EC-3
D. EC-4
Answer: C
279. Which of the following is serine protease ?
A. Pepsin
B. Trypsin
C. Carboxypeptidase
D. None
Answer: B
280. Reducing equivalants produced in glycolysis are transported from cytosol to mitochondria by ?
A. Carnitine
B. Creatine
C. Malate shuttle
D. Glutamate shuttle
Answer: C
281. HMP shunt occurs in all organs except ?
A. Liver
B. Adipose tissue
C. RBC
D. Brain
Answer: D
282. Fastest acting enzyme ?
A. LDH
B. Trypsin
C. Catalase
D. None
Answer: C
283. Inhibition of glycolysis by increased supply of 02 is called ?
A. Crabtree effect
B. Pasteur effect
C. Lewis effect
D. None
Answer: B
284. RNA is present in ?
A. Cytoplasm
B. Nucleus
C. Ribosome
D. All of the above
Answer: D
285. All are reducing sugars except
A. Sucrose
B. Lactose
C. Glucose
D. Fructose
Answer: A
286. Reducing sugar in urine can be detected by
A. Benedicts test
B. Fehling solution
C. Glucose-oxidase test
D. All of the above
Answer: D
287. Substrate level phosphorylation is by
A. Pyruvate kinase
B. Phosphofructokinase
C. Hexokinase
D. ATP synthase
Answer: A
288. Type VI glycogen storage disease is due to the deficiency of –
A. Muscle phosphorylase
B. Glucose-6-phosphatase
C. Liver phosphorylase
D. Branching enzyme
Answer: C
289. Xanthoproteic reaction involves
A. Carbolic acid
B. H2SO4
C. HCL
D. Nitric acid
Answer: D
290. Coenzyme not required in formation of glutamate
A. Thiamine pyrophosphate
B. Pyridoxial phosphate
C. Niacin
D. None of the above
Answer: A
291. Zewellger syndrone is due to absence of
A. Lysosomal
B. Mitochondria
C. Peroxisome
D. Nucleus
Answer: C
292. Urea, creatinine, nitric oxide formed by which amino acid
A. Histidine
B. Glycine
C. Cysteine
D. Arginine
Answer: D
293. Which of the following is not the source of cytosolic NADPH ?
A. Isocitrate dehydrogenase
B. ATP citrate lyase
C. Malic enzyme
D. G6PD
Answer: B
294. All are true regarding mitochondrial DNA, EXCEPT ?
A. Double stranded
B. Inherited from mother
C. High mutation rate
D. All respiratory proteins are synthesized within mitochondria
itself
Answer: D
295. Which apolipoprotein is responsible for Alzeihmers disease
A. APOE4
B. APOE3
C. APOE2
D. APOE1
Answer: A
296. Hyperammonaemia inhibit TCA cycle by depleting?
A. succinate
B. a keto glutarate
C. malate
D. fumarate
Answer: B
297. which amino acid is used to synthesise Nitric oxide ?
A. glycine
B. arginine
C. tyrosine
D. threonine
Answer: B
298. Menkes disease is associated with which enzyme deficiency.
A. lysyl oxidase
B. Methionine synthase
C. Glutamyl aminopeptidase
D. Lysyl hydroxylase
Answer: A
299. LCAT deficiency increases the following
A. HDL
B. LDL
C. VLDL
D. Chylomicron
Answer: A
300. A 25 year old alcoholic presented with edema, hypertension, ocular disturbance, and changes in mental state was observed, diagnosis of high output cardiac failure was made with Wet Beri Beri, this is due to deficiency of?
A. Vit B3
B. Vit B6
C. Vit B9
D. Vit B1
Answer: D
301. Glutamine is Increase in CSF, blood and urine, this is due to deficiency of
A. CPS-I
B. Arginase
C. OTC
D. Argininosuccinate synthetase
Answer: A
302. True about type 1 diabetes mellitus
A. Decreased protein catabolism
B. Decreased hepatic Glucose output
C. Increased lipolysis
D. Increase glucose uptake
Answer: C
303. Protein which is not synthesised In liver is
A. Phase protein
B. Immunoglobulins
C. Albumin
D. Plasma hormone
Answer: B
304. type of cholesterol present in gallstones?
A. Amorphous cholesterol monohydrate.
B. Amorphous cholesterol dihydrate.
C. Crystalline Cholesterol dihydrate.
D. Crystalline cholesterol monohydrate.
Answer: D
305. collagen present in skin is
A. Type II
B. Type I
C. Type III
D. Type IV
Answer: B
306. which of the following is not Ribozyme?
A. Ploy A polymerase
B. Ribonuclease
C. Transpeptidase
D. Peptidyl Transferase
Answer: A
307. Type-I hyperlipoproteinemia is characterized by
A. Elevated LDL
B. Elevated HDL
C. Elevated chylomicrons
D. Elevated lipoprotein lipase
Answer: C
308. In Kreb’s cycle and Urea cycle the linking amino acid is
A. Fumarate
B. Alanine
C. Arginine
D. Aspartate
Answer: D
309. The cofactor vitamin B12 is required for the following conversion:
A. Dopamine to Norepinephrine
B. Propionyl CoA to methyl malonyl CoA
C. Methyl malonyl CoA to succinyl CoA
D. Cysteine to homocysteine
Answer: C
310. Which of the following enzyme activity decreases in fasting?
A. Hormone sensitive lipase
B. Glycogen phosphorylase
C. Acetyl CoA Carboxylase
D. Phosphofructokinase I
Answer: D
311. Which micronutrient deficiency causes anemia?
A. Copper
B. Molybdenum
C. Selenium
D. Flurine
Answer: A
312. True about sigma factor?
A. Subunit of 50s ribosome
B. Subunit of DNA polymerase
C. Subunit of RNA polymerase
D. Initiates DNA replication
Answer: C
313. In ETC, cyanide inhibits ?
A. Complex I
B. Cytochrome C oxidase
C. Complex IV
D. Complex III
Answer: B:C
314. HHH syndrome is due to defect in ?
A. Tryptophan metabolism
B. Histidine transporter
C. Branched chain AA metabolism
D. Ornithine transporter
Answer: D
315. Earliest symptom of Tay sach disease
A. Exaggerated startle response
B. Bone deformation
C. Hepatomegaly
D. Excessive bleeding
Answer: A
316. Most important tool used in genetic engineering
A. Halicase
B. Topoisomerase
C. DNA Ligase
D. Restriction endonuclease
Answer: D
317. Chromosomal instability syndrome is
A. Fanconi syndrome
B. Ataxia Telangectasia
C. Bloom syndrome
D. All of the above
Answer: D
318. Vitamin B1 is required for which reaction
A. Transamination
B. Oxidative decarboxylation
C. Carboxylation
D. All of the above
Answer: B
319. Hyperammonemia type-1 is due to deficiency of
A. Arginase
B. Arginosuccinate lyase
C. Arginosuccinate synthase
D. CPS-1
Answer: D
320. Major site of protein glycosylation is ?
A. ER and golgi body
B. Ribosome and golgi body
C. ER and ribosome
D. Ribosome and cytoplasm
Answer: A
321. Which is not a step of PCR ?
A. Annealing
B. Extension
C. Transformation
D. Denaturation
Answer: C
322. Which does not play a role in protein synthesis?
A. Exon
B. Intron
C. m-RNA
D. ATP
Answer: B
323. Pyridoxine deficiency leads to altered metabolism of?
A. Phenylalanine
B. Tryptophan
C. Methionine
D. Tyrosine
Answer: B
324. Kcat/km is a measure of
A. Enzyme efficiency
B. Speed of enzymatic reaction
C. Concentration of substrate
D. Enzyme turn over
Answer: D
325. Enzyme involved in the transfer of hydrogen ion is
A. Hydratase
B. Oxidase
C. Peroxidase
D. Dehydrogenase
Answer: B😀
326. Which of the following is a constitutive enzyme?
A. Hexokinase
B. Glucokinase
C. p galactosidase
D. Cyclooxygenase-2
Answer: A
328. Regarding energy production by the electron transport chain, which is true?
A. The complexes are arranged in a decreasing order of redox potential
B. The complexes are arranged in a decreasing order of ability to get reduced
C. The complexes are arranged in a decreasing order of state of oxidation
D. The complexes are arranged in a decreasing order of energy level
Answer: D
329. Electron transport chain all are true except
A. Complexes are arranged in an increasing order of redox potential;
B. Mitochondrial Glycerol phosphate dehydrogenase sends its electron directly to Q
C. l0 Hydrogen ions are translocated when NADH enters into an electron transport chain
D. 7 Hydrogen ions are translocated when FADH2 electrons get into electron transport chain.
Answer: D
330. Which of the following is the respiratory centre of cell?
A. Mitochondria
B. Microsome
C. Lysosome
D. Nucleus
Answer: A
331. Cellulose is biochemically
A. ß (1,4) L glucose
B. ?, (14) D glucose
C. ß(1,4) D glucose
D. ? (1,4) L glucose
Answer: A
332. D and L isomerism is
A. Optical isomerism
B. Functional isomerism
C. Epimerism
D. Enantiomerism
Answer: D
333. All of the following are converted to cr-ketoglutarate on catabolism except
A. Glutamate
B. Histidine
C. Proline
D. Glycine
Answer: D
334. Glucogenic aminoacids give rise to all of the following intermediates of citric acid cycle except
A. Isocitrate
B. c ketoglutarate
C. Succinyl CoA
D. Fumarates
Answer: A
335. What is the precursor of proline in Krebs cycle?
A. Oxaloacetate
B. c ketoglutarate
C. Succinyl CoA
D. Fumarates
Answer: B
336. used in citric acid cycle are all except
A. NAD
B. FAD
C. NADP
D. GDP
Answer: C
337. All of the following steps act as sources of energy in citric acid cycle except
A. Citrate synthase
B. Isocitrate dehydrogenase
C. Succinyl Thiokinase
D. Succinate Dehydrogenase
Answer: A
338. True about glucokinase is
A. It is present in all cells
B. It is a constitutive enzyme
C. It has a high km
D. It is inhibited by glucose 6 phosphate
Answer: C
339. All of the following are true about lactate utilisation in liver except
A. Total net number of ATP formed because of cori’s cycle is 6
B. Cori’s cycle shifts the metabolic burden from muscle to liver
C. Cori’s cycle can not be sustained indefinitely because it is energetically unfavourable
D. Cori’s cycle is linked to glycogen synthesis in muscle
Answer: A
340. Which of the following is true about effect of insulin and glucagon on gluconeogenesis?
A. Insulin favours the formation of fructose 2,6 bisphosphate
B. Fructose 2, 6 bisphosphate is an inhibitor of glycolysis
C. Insulin acts through a kinase
D. Glucagon stimulates PFK.2 activity of the tandem enzyme
Answer: A
341. In glycogen synthesis the active form of glucose used is
A. Glucose 6 phosphate
B. Glucose I phosphate
C. UDP glucose
D. UTP glucose
Answer: C
.
342. Neonatal hypoglycaemia which does not respond to counter regulatory hormone administration is diagnostic of
A. Her’s disease
B. Cori’s disease
C. Anderson’s disease
D. Von Gierke’s disease
Answer: D
343. Which of the following is a transfatty acid?
A. Oleic acid
B. Elaidic acid
C. Stearic acid
D. Arachidonic acid
Answer: B
344. Activators of Acetyl CoA carboxylase are all except
A. Acyl coA
B. Citrate
C. Glutamate
D. Dicarboxylic acid
Answer: A
345. Arachidonic acid oxidation involves how many cycles of beta oxidation?
A. l0
B. 20
C. 9
D. 8
Answer: C
346. All are true about beta oxidation of fatty acids except
A. Carnitine acyl transferase I is the rate limiting enzyme of fatty acid oxidation
B. Carnitine acyl transferase I is stimulated by Acyl CoA
C. Carnitine Acyl transferase I is stimulated by malonyl CoA
D. Carnitine Acyl transferase I defect causes a decrease in
acylcarnitine levels
Answer: C
347. AII are true about beta oxidation of fats acids except
A. Occurs in mitochondria
B. Occurs in peroxisome
C. Results in hydrogen peroxide generation
D. Fatty acid oxidation defects present with ketosis
Answer: D
348. Which of the following is true about Beta oxidation of fatty acids?
A. Stearic acid on oxidation provides 106 ATPs
B. Odd chain fatty acid oxidation provides only propionyl coA
C. Fatty acid oxidation defects cause hypoglycemia
D. Ketone bodies are formed by incomplete oxidation of fatty acid during starvation to increase energy production
Answer: C
349. Which of the following is true about properties of VLDL/LDL
A. In electrophoresis, VLDL migrates more cathodal than LDL
B. LDL is formed from liver
C. LDL is formed from Chylomicron
D. VLDL remnants reach extrahepatic tissues
Answer: B
350. Reverse cholesterol transport – all are true except
A. Transport of cholesterol from extrahepatic tissues to liver
B. ATP Binding Cassette Transporter protein is involved in the conversion of HDL3 to HDL2
C. Lecithin Cholesterol Acyl Transferase helps in the conversion of Spheroidal HDL to Discoidal HDL
D. Cholesterol Ester Transfer Protein helps in increasing HDL level
Answer: D
351. True about coproporphyrin I and coproporphyrin III is
A. Coproporphyrin I is excreted in urine
B. Coproporphyrin III is excreted in bile
C. In Dubin Johnson Syndrome, Coproporphyrin I in urine is 80% of the total coproporphyrin
D. In Dubin Johnson Syndrome, total coproporphyrin levels is elevated
Answer: C
352. Synthetic oxygen carrier is ?
A. 2,4 dinitrophenol
B. Chloflurocarbon
C. Perflurocarbon
D. 1 fluro 2,4 dinitrophenol
Answer: B
353. Abetalipoproteinemia affects ?
A. Retinal pigment epithelium
B. Optic nerve
C. Occipital cortex
D. Bipolar neurons
Answer: A
354. Genetically mediated VLDL overproduction is a feature of all except ?
A. Familial combined hyperlipidemia
B. Hypoapobetalipoproteinemia
C. Familial dyslipidemic hypertension
D. LDL subclass B
Answer: B
355. Gerhardt’s test is used to detect ?
A. Reducing sugar
B. Ketone body
C. Protein
D. Blood
Answer: B
356. Folds in collagen are due to
A. Glycine
B. Proline
C. Hydroxyproline
D. Lysine
Answer: A:B
357. Progesterone synthesis requires
A. LDL
B. VLDL
C. HDL
D. Chylomicron
Answer: C
358. The most abundant aminoacid of collagen is
A. Glycine
B. Proline
C. Lysine
D. Tryptophan
Answer: A
359. Site of small chain fatty acid absorption is
A. Ileum
B. Duodenum
C. Ascending colon
D. Rectum
Answer: C
360. All of the following are true about collagen structure except
A. Collagen is secreted by fibroblasts as procollagen
B. Lysyl oxidase is dependent on Vitamin C
C. Hydroxylysine undergoes glycosylation
D. Glycine is the most abundant aminoacid of collagen
Answer: B
361. HMG CoA is precursor of all except
A. Ubiquinone
B. Dolichol
C. Bile pigments
D. Ketone body
Answer: C
362. Small RNAs are ?
A. Between 200 and 500 bps in length
B. Coded by small exons
C. A mode of regulation of gene expression
D. Always endogenously synthesised
Answer: C
363. What is the parameter that is used to assess lipid peroxidation?
A. Malondialdehyde
B. CRP
C. hsCRP
D. Carboxymethyl lysine
Answer: A
364. Ubiquitin Proteasome pathway is used for degradation of ?
A. Extracellular long lived proteins
B. Intracellular long lived proteins
C. Intracellular short lived proteins
D. Extracellular short lived proteins
Answer: C
365. Amino acid in synthesis of neurotransmitter
A. Glutamate
B. Proline
C. Cysteine
D. Alanine
Answer: A
366. Maximum buffering capacity of a buffer is maximum at pH
A. Less than pka
B. More than pka
C. Equal to pka
D. Has no relation with pka
Answer: C
367. LacY in Lac Operon codes for ?
A. B Galactosidase
B. Galactoside Permease
C. Thiogalactoside Transacetylase
D. Repressor
Answer: B
368. Essential amino-acid deficiency affect nitrogen balance by
A. Increasing protein degradation
B. Decreasing protein degradation
C. Decreasing protein synthesis
D. Increasing protein synthesis
Answer: C
369. Function of tyrosinase is
A. Synthesis of norepinephrine
B. Synthesis of dopamine
C. Synthesis of melanin
D. All of the above
Answer: C
370. Chimeric DNA true are all except ?
A. Formed by linking DNA fragments of unrelated genome
B. Sticky end producing restriction endonucleases favour formation of chimeric DNA
C. They don’t require DNA ligases
D. The organism harbouring a chimeric DNA has features of themselves and the properties of the insert
Answer: C
371. Enzyme which acts on aspartate
A. Serum Glutamate Pyruvate Transaminase (SGPT)
B. Serum Glutamate Oxaloacetate Transaminase (SGOT)
C. Ornithine transcarbmaylase (OTC.
D. Argininosuccinate lyase (ASL)
Answer: B
372. Proteins are stored as ?
A. Structural proteins
B. Functional proteins
C. Fats
D. Lysosomal enzymes
Answer: C
373. Allosteric stimulator of glutamate dehydrogenase is
A. ATP
B. GTP
C. Palmitoyl CoA
D. Leucine
Answer: D
374. Aminoacyl tRNA gets attached to which site of ribosome?
A. P site of 40s ribosome
B. A site of 60s ribosome
C. A site of 40s ribosome
D. P site of 60s ribosome
Answer: B
375. Which of the following is a biologically important tripeptide?
A. Thyrotropin releasing hormone
B. Thyroid stimulating hormone
C. Gonadotropin releasing hormone
D. Follicle Stimulating hormone
Answer: A
376. Left handed helix is seen in
A. B DNA
B. A DNA
C. Z DNA
D. F DNA
Answer: C
377. DNA fragments are separated by ?
A. Ultracentrifugation
B. Agarose gel electrophoresis
C. Paper chromatography
D. High pressure liquid chromatography
Answer: B
378. Carbamoyl Phosphate synthetase I [CPSI] true is
A. It is present in cytoplasm
B. It is involved in pyrimidine synthesis
C. N- Acetyl Glutamate is an allosteric stimulator of CPSI
D. Glutamine is the amino group donor for CPSI
Answer: C
379. Molecular mimicry is established in the presence of ?
A. Cysteine
B. Alanine
C. Glycine
D. Tryptophan
Answer: A
380. All of the following can determine protein structure except
A. Edman’s Sequencing
B. X ray crystallography
C. Optical rotatory dispersion
D. Spectrophotometry
Answer: D
381. Edman’s reagent is used for
A. DNA sequencing
B. Protein sequencing
C. Protein Denaturation
D. DNA denaturation
Answer: B
382. Most abundant aminoacid in brain is
A. Glutamate
B. Aspartate
C. Glutamine
D. Asparagine
Answer: A
383. Cystine has how many molecules of cysteine?
A. 1
B. 2
C. 3
D. 4
Answer: B
384. Jumping genes are ?
A. Moderately repetitive sequences
B. Highly repetitive sequences
C. Satellite sequences
D. Simple segment repeat sequences
Answer: A
385. Vitamin given in homocysteinuria are all except
A. Vitamin B6
B. Vitamin B12
C. Folate
D. Thiamine
Answer: D
386. Glutathione is used to detoxify which free radical?
A. Hydrogen peroxide
B. Superoxide
C. Peroxyl radical
D. Singlet Oxygen
Answer: A
387. Ataxia telangiectasia is caused by a defect of ?
A. Base Excision Repair
B. Nucleotide Excision repair
C. Mismatch repair
D. ds DNA break repair
Answer: D
388. Bond formation between ribose sugar and nitrogen is ?
A. Acidanhydride linkage
B. Phosphodiester linkage
C. Phosphoester linkage
D. Glycosidic linkage
Answer: D
389. The linkage which links individual nucleotides in a polynucleotide chain is
A. p N- Glycosidic linkage
B. a N – glycosidic linkage
C. 3’5′ Phosphodiester linkage
D. 5’3′ Phosphodiester linkages
Answer: C
390. All of the following are true about Nucleic Acid Sequence Based Amplification except ?
A. It is a specific amplification of RNA
B. It is a replacement for reverse transcriptase PCR
C. Denaturation is carried out at 94°C
D. It requires Reverse transcriptase.
Answer: C
391. Regarding FISH all are true except ?
A. Used to detect copy number variations
B. Used to detect balanced translocations
C. Requires oligonucleotides
D. Requires DNA polymerase
Answer: D
392. True about pyrimidine catabolism is ?
A. It is a source of uric acid
B. ß aminoisobutyrate is generated
C. Unlike other catabolic pathways, it does not generate intermediates of citric acid cycle
D. Increased pyrimidine catabolism causes decreased synthesis of carnosine
Answer: B
393. True about Purine synthesis ?
A. Glutamine is the amino group donor for N9
B. PRPP synthetase is the rate limiting enzyme of purine synthesis
C. THFA is necessary for forming C6 of purine ring
D. GMP is the first nucleotide to be formed during purine synthesis
Answer: A
394. Antistress Vitamin is?
A. Vitamin B1
B. Vitamin B2
C. Vitamin B3
D. Vitamin B5
Answer: D
396. Regarding NAD and NADP, true is ?
A. Precursor is tyrosine
B. Malic enzyme is an NAD dependent enzyme
C. High leucine causes niacin deficiency
D. Niacin deficiency causes cutaneous vasodilatation
Answer: C
397. Which of the following has two amino groups
A. Glycine
B. Arginine
C. Lysine
D. Asparagine
Answer: B
398. Deficiency of which vitamin during pregnancy predisposes to meningomyelocele?
A. Folic acid
B. Biotin
C. Pyridoxine
D. Thiamine
Answer: A
399. Vitamin B12 deficiency causes all except ?
A. Homocysteinuria
B. Methylmalonic aciduria
C. Subacute combined degeneration
D. Epinephrine excess
Answer: D
400. Alternate fuel for brain is
A. Glucose
B. Ketone bodies
C. Fatty acid
D. Aminoacid
Answer: B
401. Pyruvate can be a substrate of all except
A. Lactate Dehydrogenase
B. Malic enzyme
C. Aspartate transaminase
D. Alanine transaminase
Answer: C
402. Which of the following elements is known to influence the body’s ability to handle oxidative stress?
A. Fluride
B. Iron
C. Copper
D. Selenium
Answer: D
403. Gas released from oligosaccharide metabolism by intestinal bacteria is
A. Carbondioxide
B. Sulphur dioxide
C. Nitric oxide
D. Methane
Answer: D
404. Chain breaking antioxidants are all except
A. Tocopherol
B. Ascorbic acid
C. Polyphenolic flavinols
D. Superoxide dismutase
Answer: D
405. Which vitamin is required for conversion of serine to glycine?
A. Vit C
B. B12
C. Pyridoxine
D. Thiamine
Answer: C
406. Which of the following vitamins is significantly synthesised in gut by intestinal flora?
A. Folate
B. B12
C. Biotin
D. B6
Answer: A:B:C
407. Headache and papilledema are features of toxicity of which vitamin?
A. Vitamin A
B. Vitamin D
C. Vitamin C
D. Vitamin E
Answer: A
408. Vitamin B12 is required for all of the following except ?
A. Conversion of homocysteine to methionine
B. Conversion of homocysteine to cysteine
C. Conversion of propionyl coA to succinyl CoA
D. Conversion of methyl THFA to THFA
Answer: B
409. Alcoholism leads to deficiency of which vitamin ?
A. Vitamin A
B. Vitamin B1
C. Vitamin D
D. Vitamin B6
Answer: B
410. AST/ALT > 2 occurs in deficiency of
A. Glucose-6-phosphotase
B. Branching enzyme
C. Acid maltase
D. Liver phosphorylase
Answer: C
411. LDH has how many isoenzymes
A. 3, based on B and M polypeptide subunits
B. 5, based on B and M polypeptide subunits
C. 7, based on H and M polypeptide subunits
D. 5, based on H and M polypeptide subunits
Answer: D
412. Example of allosteric inhibition
A. Inactivation of glycogen synthase by phosphorylation
B. Decreased synthesis of glucokinase by glucagon
C. Inhibition of PFK-1 by citrate
D. All of the above
Answer: C
413. Lysyl oxidase requires which cofactor
A. Zn
B. Cu
C. Se
D. Fe
Answer: B
414. Mechanism of conversion of trypsinogen to trypsin
A. Hydrolysis
B. Phosphorylation
C. Removal of part of protein
D. Removal of Carboxyl group
Answer: C
415. True about NADP
A. Acts as coenzyme form of Niacin
B. Involved in HMP shunt
C. Not involved in glycolysis
D. All are true
Answer: D
416. In malate shuttle, NADH Produces how many ATPs
A. 1
B. 1-5
C. 2
D. 2.5
Answer: D
417. Pyruvate dehydrogenase requires all cofactors except
A. Thiamin
B. Riboflavin
C. Niacin
D. Pyridoxin
Answer: D
418. Lactate produced anaerobically is used by
A. Gluconeogenesis & Glycolysis
B. Cori cycle & gluconeogenesis
C. TCA cycle & Glycogenolysis
D. Cori cycle only
Answer: B
419. Citrate synthase is inhibited by
A. ATP
B. ADP
C. Insulin
D. Glucagon
Answer: A
420. Fumarate is formed from which amino acid
A. Methionine
B. Valine
C. Histidine
D. Tyrosine
Answer: D
421. The major role of 2, 3 bisphosphoglycerate in RBCs is
A. Acid-base balance
B. Reversal of glycolysis
C. Release of oxygen
D. Binding of oxygen
Answer: C
422. Enzyme deficient in Hers disease
A. Muscle phosphorylase
B. Liver phosphorylase
C. Acid maltase
D. Debranching enzyme
Answer: B
423. Immediate metabolic products during conversion of Fructus 1-6 bisphosphate to 2 molecules of pyruvate
A. Glyceraldehyde-3-phosphate and 1,3-bisphosphoglycerate
B. Dihydroxyacetone phosphate and 1,3 bisphos?phoglycerate
C. Glyceraldehyde-3-phosphate and dihydroxy-acetone phosphate
D. 3-phosphoglycerate and 1,3 bisphosglycerate
Answer: C
424. In conversion of glucose to glucose-6phosphate in glycolysis true is
A. Glucokinase has low km
B. Hexokinase is found only in liver
C. Glucokinase is induced by insulin
D. Hexokinase is not specific for glucose
Answer: C:D
425. Major carbohydrate store in the body
A. Blood glucose
B. Glycogen in adipose tissue
C. Hepatic glycogen
D. None of the above
Answer: C
426. Most abundant source of fuel in starvation
A. Liver glycogen
B. Muscle glycogen
C. Adipose tissue
D. Blood glucose
Answer: C
427. Major metabolism of saturated fatty acids in the mitochondria is called as
A. ß-oxidation
B. a-oxidation
C. ?-oxidation
D. None of the above
Answer: A
428. Which method is used to separate a mixture of lipids
A. Electrophoresis
B. Chromatography
C. Isoelectric focusing
D. PAGE
Answer: B
429. Chylomicrons core is formed by ?
A. Triglyceride
B. Triglyceride and Cholesterol
C. Triglyceride, Cholesterol and Phospholipids
D. Free fatty acids
Answer: B
430. All are true about Niemann- Pick disease except
A. Due to deficiency of sphingomyelinase
B. CNS symptoms in type A
C. Histiocytes showing PAS positive inclusions and Type B is less
severe
D. None
Answer: D
431. Acetyl CoA Carboxylase is stimulated by
A. Starvation
B. Glucagon
C. Citrate
D. All of the above
Answer: C
432. Cholesterol is not a precursor for synthesis of
A. Vitamin D
B. Progesterone
C. Bile acids
D. Lipocortin
Answer: D
433. HDL is called good cholesterol because
A. Removes cholesterol from extrahepatic tissues
B. Causes transport of cholesterol to extrahepatic tissues
C. Stimulate hepatic TGs synthesis
D. Activates lipoprotein lipase
Answer: A
434. Amide group is present in which part of protein
A. Amino-terminal
B. Carboxy-terminal
C. Peptide bond
D. Disulfide bond
Answer: C
435. Non- essential amino-acids are all except
A. Basic amino acids
B. Acidic amino acids
C. Neutral amino acids
D. None of the above
Answer: A
436. Amino acid which can be used in both gluconeogenesis and ketogenesis
A. Threonine
B. Valine
C. Tyrosine
D. Arginine
Answer: C
437. In phenylketonuria, diet restriction is advised for
A. Tyrosine
B. Phenylalanine
C. Maize
D. All
Answer: B
438. Guanidinium group is associated with
A. Tyrosine
B. Arginine
C. Histidine
D. Lysine
Answer: B
439. Coenzyme for phenylalanine hydroxylase is
A. Tetrahydrofolate
B. Pyridoxal phosphate
C. S-adenosyl methionine
D. Tetrahydrobiopterin
Answer: D
440. Which of the following is required in the synthesis of acetylcholine
A. Inositol
B. Carnitine
C. Glycine
D. Choline
Answer: D
441. Most important factor which causes lactic acidosis in alcoholics
A. Pruduction of NADH
B. Formation of acetaldehyde
C. Production of acetate
D. None of the above
Answer: A
442. Insulin dependant cells are
A. Pituitocytes
B. Myocytes
C. Adipocytes
D. RBCs
Answer: B:C
443. Epinephrine increases free fatty acid level by causing
A. Increased fatty acid synthesis
B. Increasing lipolysis
C. Increasing cholesterol catabolism
D. All of the above
Answer: B
444. Vitamin formed in the body
A. B 1
B. B 3
C. B 6
D. B 12
Answer: A:B:D
445. Which of the following is not seen in 12 days of fasting
A. Gluconeogenesis
B. Ketogenesis
C. Lipolysis
D. Glycolysis
Answer: D
446. Pyridoxine is required in
A. Glycolysis
B. TCA cycle
C. Glycogenesis
D. Glycogenolysis
Answer: D
447. Pruritis [Itching] is caused by deficiency of
A. HMB synthase
B. 5-ALA dehydratase
C. Uroporphyrinogen – I synthase
D. Uroporphyrinogen – III synthase
Answer: D
448. Vitamin acting on intranuclear receptors
A. Vitamin K
B. Vitamin D
C. Vitamin E
D. Vitamin E
Answer: B
449. Niacin acts as coenzyme A. TPP
B. FAQ
C. NAD
D. NAD
Answer: C
450. Not a metabolic product of urea cycle
A. Citrulline
B. Ornithine
C. Alanine
D. Arginine
Answer: C
451. Which amino acid is not involved in transamination
A. Alanine
B. Aspartate
C. Lysine
D. Histidine
Answer: C
452. Apo B48 is synthesized in
A. Liver
B. Kidney
C. Intestine
D. RBCs
Answer: C
453. What are okazzaki fragments
A. Long pieces of DNA on lagging strand
B. Long pieces of DNA on leading strand
C. Short pieces of DNA on lagging strand
D. Short pieces of DNA on leading strand
Answer: C
454. C4, C5, N7 in purine ring are derived from
A. Aspartate
B. Glutamine
C. Glutamine
D. CO
Answer: C
455. If content of A is 15%, what is the amount of G in DNA according to Chargaff s rule?
A. 15%
B. 85%
C. 35%
D. 70%
Answer: C
456. cDNA from RNA is synthesized by
A. Helicase
B. DNA dependent DNA polymerase
C. Topoisomerase
D. Reverse transcriptase
Answer: D
457. Which is not a chaperon protein
A. Calnexin
B. Protein disulfide isomerase
C. Calreticulin
D. Calbindin
Answer: D
458. All are functions of glycosaminglycans except
A. Lubrication
B. Wound healing
C. Anticoagulant
D. Transport of lipids
Answer: D
459. Bile salts help in absorption of fat by
A. Micelles formation
B. Activation of transporter protein
C. Creation of concentration gradient
D. All of the above
Answer: A
460. a-aminolevulinic acid is a metabolic product in synthesis of
A. Tryptophan
B. Collagen
C. Glycosaminoglycans
D. Heme
Answer: D
461. Which is an inhibitor of ferrochelatase ?
A. Lead
B. Mercury
C. Iron
D. Arsenic
Answer: A
462. Bile acids consist of all except
A. Lithocholic acid
B. Taurocholic acid
C. Deoxycholic acid
D. Chendeoxycholic acid
Answer: B
463. True about cAMP and cGMP
A. Second messengers
B. Act on membrane receptors
C. Act by post-translational modification
D. All of the above
Answer: D
464. Spectroscopy is used for interaction of
A. Electromagnetic radiation
B. Protons
C. Alpha particles
D. Positrons
Answer: A
465. Abnormal proteins which are bound to ubiquitin are degraded in
A. Proteosomes
B. Golgi apparatus
C. Smooth ER
D. Lysosomes
Answer: A
466. Enzyme degradation is caused by
A. Ubiquitin
B. RNAse
C. Zymase
D. Chaperone
Answer: A
467. G1cNAc-P-P- oligosacharride is
A. Proteoglycan
B. Glycoprotein
C. Collagen
D. Phospholipid
Answer: B
468. Prolyl hydroxylase require which cofactor
A. Sc
B. Vitamin C
C. Mo
D. Vitamin K
Answer: B
469. Side chain linkage in proteoglycons
A. Covalent
B. Hydrogen bond
C. Electrostatic bond
D. Van-der Waal’s force
Answer: A
470. Which of following is not a free radical
A. H202
B. (M?
C. Superoxide anion
D. HOC1-
Answer: D
471. In type la maple syrup urine disease, gene mutation seen is ?
A. Ela
B. Elb
C. E2
D. E3
Answer: A
472. Hexokinase is inhibited by ?
A. Glucose-6-phosphate
B. Glucagon
C. Glucose
D. Insulin
Answer: A
473. All of the following are inhibited during fasting/ starvation, except ?
A. Hexokinase
B. Glucokinase
C. PDH
D. Pyruvate kinase
Answer: A
474. Hereditary orotic aciduria Type-I is due to deficiency of ?
A. Orotate phosphoribosyl transferase
B. Orotic acid decarboxylase
C. UMP synthase
D. All of the above
Answer: D
475. Replication of mitochondrial DNA is caused by which type of DNA polymerase ?
A. a
B. p
C. S
D. y
Answer: D
476. Which isoform of LDH is raised in Anemia ?
A. LDH 5
B. LDH 4
C. LDH 3
D. LDH 2
Answer: D
477. Digestive enzymes are
A. Hydrolases
B. Oxidoreductases
C. Dehydrogenases
D. Ligases
Answer: A
478. Enzymes that move a molecular group from one molecule to another are known as
A. Ligases
B. Oxido-reductases
C. Transferases
D. Dipeptidases
Answer: C
479. Glucose-6-phosphate dehydrogenase need
A. NAD
B. NADP
C. FAD
D. FMN
Answer: B
480. Which of the following is NADP linked
A. G6PD
B. APDH
C. a-keto glutarate dehydrogenaes
D. None
Answer: A
481. Oxidative deamination occurs in ?
A. Cytoplasm of all cells
B. Mitochondria of all cells
C. Cytoplasm of hepatocytes
D. Mitochondria of hepatocytes
Answer: D
482. Aldehyde dehydrogenase requires NAD as ?
A. Cofactor
B. Apoenzyme
C. Coenzyme
D. None
Answer: C
483. In FITC the color emitted after blue light absorption?
A. Yellow green
B. Orange red
C. Apple green
D. Golden brown
Answer: A
484. Apoenzyme is ?
A. Cofactor
B. Coenzyme
C. Protein moiety
D. None
Answer: C
485. In xanthine oxidase co factor is ?
A. Selenium
B. Zn
C. Molybdenum
D. Mg
Answer: C
486. What are isoenzyme
A. Physically same forms of different enzymes
B. Physically disinct forms of same enzyme
C. Forms of same enzyme that catalyze different reactions
D. Forms of different enzyme that catalyze same reactions
Answer: B
487. Enzyme causing covalent bond cleavage without hydrolysis ?
A. Lyase
B. Ligase
C. Hydrolase
D. Transferase
Answer: A
488. Enzymes act by ?
A. Increase in activation energy
B. Decrease in activation energy
C. Shift equilbrium constant
D. None
Answer: B
489. Shortest peptide ?
A. Angiotensin II
B. Angiotensin III
C. Oxytocin
D. Vasopressin
Answer: B
490. The predominant isoenzyme of LDH occuring in liver injury is ?
A. LDH-1
B. LDH-2
C. LDH-4
D. LDH-5
Answer: D
491. Specific activity of enzyme is ?
A. limo’ of enzyme per gram of substrate
B. Enzyme units per mg of protein
C. Conc. of substrate transformed per minute
D. None
Answer: B
492. Type of inhibition of aconitase by Transaconitate is?
A. Non-competitive
B. Competitive
C. Allosteric
D. None
Answer: B
493. Functional plasma enzyme is ?
A. Fibrinogen
B. LDH
C. SGOT
D. SGPT
Answer: A
494. NADPH via glycerophosphate shunt makes how many ATP?
A. 1
B. 2
C. 3
D. 4
Answer: B
495. Which of the following tripeptide ?
A. Glutathione
B. Angiotensin
C. Glucagon
D. Oxytocin
Answer: A
496. Enzyme involved in oxidative phosphorylation ?
A. Pyruvate kinase
B. Succinyl CoA thiokinase
C. NADH dehydrogenase
D. None
Answer: C
497. Protein segregation occurs in ?
A. Golgi appparatus
B. Peroxisomes
C. ER
D. Mitochondria
Answer: A
498. Weakest bond is ?
A. Covalent
B. Hydrogen
C. Electrostatic
D. Vander wall
Answer: D
499. In ETC NADH generates
A. 1 ATPs
B. 2 ATPs
C. 3 ATPs
D. 4 ATPs
Answer: C
500. This attaches to protein before destruction ?
A. Ubiquitin
B. RNAseF
C. Zymase
D. Chaperone
Answer: A
501. In glycolysis, inorganic phosphate is used reaction, catalyzed by ?
A. Enolase
B. Pyruvate kinase
C. Glyceraldehyde-3-p dehydrogenase
D. Aldolase
Answer: C
502. Protein glycosylation occurs in ?
A. ER
B. Golgi bodies
C. Mitochondria
D. Peroxisomes
Answer: A:B
503. True about glycolysis?
A. Hexokinase produce ATP
B. 1 cycle produces 2 ATP
C. It produces directly 2 molecules of lactate
D. Aldolase produces irreversible polymerization
Answer: C
504. The rate limiting step in glycolysis is catalyzed by?
A. Pyruvate kinase
B. Enolase
C. Glucokinase
D. Phosphofructokinase
Answer: A:C:D
505. Rate limiting enzyme in heme synthesis ?
A. ALA synthase
B. Hmg coa reductase
C. ALA dehydratase
D. Uroporphyrinogen 1 synthase
Answer: A
506. Reverse folding of proteins is carried out by ?
A. Valine
B. Threonine
C. Chaperone
D. Aspartate
Answer: C
507. Myoglobin contains ?
A. Iron
B. Copper
C. Zink
D. Selenium
Answer: A
508. All are activated by insulin except ?
A. Lipoprotein lipase
B. Pyruvate kinase
C. Hormone sensitive lipase
D. Acetyl-CoA carboxylase
Answer: C
509. Acute intermittent porphyria is due to deficiency of?
A. Uroporphyrinogen I synthase
B. Uroporphyrinogen III synthase
C. Ferrochelatase
D. ALA synthase
Answer: A
510. Nonsense codons bring about ?
A. Elongation of polypeptide chain
B. Pre-translational modificastion of protein
C. Initiation of protein synthesis
D. Termination of protein synthesis
Answer: D
511. Repetitive chains of glucosamine with uronic acid are seen in ?
A. NANA
B. Heparan sulphate
C. Keratan sulphate
D. None of these
Answer: B
512. ATP is consumed at which step of glycolysis
A. Enolase
B. Hexokinase
C. Pyruvate kinase
D. Isomerase
Answer: B
513. Which of the following GAG is not sulphated ?
A. Chondroitin
B. Dermatan
C. Keratan
D. Hyaluronic acid
Answer: D
514. Methionine can entre the TCA cycle at which level?
A. Fumarate
B. Oxabacetate
C. Succinyl – CoA
D. Citrate
Answer: C
515. Rate limiting steps in TCA cycle ?
A. a–Ketoglutarate Succinyl CoA
B. Citrate – Isocitrate
C. Succinyl CoA 4 Succinate
D. Succinate – Fumarate
Answer: A
516. The folds in collagen is due to ?
A. Glycine
B. Alanine
C. Arginine
D. Histidine
Answer: A
517. Rate limiting step in TCA cycle is catalyzed by
A. a-ketoglotarate synthase
B. Fumarase
C. Aconitase
D. Thiokinase
Answer: A
518. Which of the following statements regarding mature cytoplasmic messenger RNA is true ?
A. Transcribed from Nuclear DNA
B. Has Thiamine in place of Uracil
C. Sugar is Deoxy Ribose
D. Its molecular weight is more than hn-RNA
Answer: A
519. Fluroacetate inhibits ?
A. Citrate synthetase
B. Aconitase
C. Succinate dehydrogenase
D. Alphaketoglutarate dehydrogenase
Answer: B
520. Identification of individed by their DNA was invented by ?
A. Shapiro
B. Lewis
C. Jeffreys
D. Pasture
Answer: C
521. Inhibitors of glycolysis are all except ?
A. Fluoride
B. Fluoroacetate
C. Arsenite
D. Iodoacetate
Answer: B
522. Which of following is an analouge guanosine ?
A. Abacavir
B. Allopurinol
C. Bromodeoxyuridine
D. None
Answer: A
523. DNA microarrays allow detection of Gene mutations using?
A. Polymerase chain Reaction
B. Cloning
C. Southern Blotting
D. Hybridization
Answer: D
524. The biosynthesis of the enzyme pyruvate carboxylase is repressed by ?
A. Insulin
B. Glucagon
C. Cortisol
D. Epinephrine
Answer: A
525. Termination codon is ?
A. AUG
B. UAA
C. AUA
D. AGG
Answer: B
526. Where can glucose 6 phosphate not be converted to glucose ?
A. Muscle
B. Liver
C. Adipose tissue
D. Kidney
Answer: A
527. Common intermediate between gluconeogenesis and fatty acid synthesis ?
A. Glucose-6-phosphate
B. Acetyl-CoA
C. Citrate
D. Succinyl-CoA
Answer: C
528. Chromosomal study is best carried out in ?
A. Prophase
B. Metaphase
C. Telophase
D. Anaphase
Answer: B
529. Glycogen synthase is activated by ?
A. Insulin
B. Glucagon
C. Epinophrine
D. AMP
Answer: A
530. Which of the following usually require a RNA intermediate for cloning/replication?
A. Transposons
B. Plasmids
C. Phages
D. Cosmids
Answer: A
531. Glycogen storage disease which presents as lysosomal storage disease ?
A. Von gierke’s disease
B. Pompes disease
C. Mcardle’s disease
D. Andersen’s disease
Answer: B
532. In sickle cell anemia, translocation on codon 6 is due to substitution of ?
A. Valine for glutamate
B. Glutamate for valine
C. Isoleucine for valine
D. Valine for isoleucine
Answer: A
533. Aldolase-B is involved in metabolism of ?
A. Galactose
B. Fructose
C. Sucrose
D. None
Answer: B
534. In humans, ascorbic acid cannot be synthesized because of ?
A. Deficiency of G6PD
B. Deficiency of xylulose kinase
C. Deficiency of L-gulonolactone oxidase
D. Deficiency of phosphoglucomutase
Answer: C
535. Function of exonuclease
A. Polymerization
B. Proof reading
C. Chain elongation
D. Termination
Answer: B
536. Glucose oxidase converts glucose to?
A. Gluconic acid
B. Glucuronic acid
C. Iduronic acid
D. Galactic acid
Answer: A
537. Glucose is converted to glucuronate by ?
A. Oxidation of aldehyde group
B. Oxidation of terminal alcohol
C. Oxidation of both
D. None
Answer: B
538. Enzyme require for cutting the strand DNA synthesis?
A. DNA polymerase
B. DNA ligase
C. Topoisomerase
D. Helicase
Answer: C
539. Increased uric acid levels are seen in which glycogen storage disease ?
A. Type I
B. Type II
C. Type III
D. Type IV
Answer: A
540. Gulonate dehydrogenase requires ?
A. NADP
B. NAD
C. FAD
D. FMN
Answer: A
541. Reverse transcription involves ?
A. RNA dependent DNA synthesis
B. DNA dependent RNA synthesis
C. DNA dependent DNA synthesis
D. RNA dependent RNA synthesis
Answer: A
542. Mutation in GLUT-2 causes ?
A. Dandy walker syndrome
B. Fanconi becker syndrome
C. Beckwith syndrome
D. Menke’s disease
Answer: B
543. Amino sugar are formed forms ?
A. Glucose- 1 -phosphate
B. Glucose-6-phosphate
C. Fructose- 1 -phosphate
D. Fructose-6-phosphate
Answer: D
544. Difference between ganglioside & cerebroside, all except?
A. Charge
B. Presence of NANA
C. Presence of carbohydrate
D. Native tissue
Answer: C
545. In glycolysis, NADH is produced at ?
A. Pyruvate kinase
B. Enolase
C. Glyceraledehyde-3-P-dehydrogenase
D. PFK-1
Answer: C
546. 1″ acetyl group donor in fatty acid synthesis is ?
A. Malonyl CoA
B. Palmitate
C. Acetyl CoA
D. Citrate
Answer: C
547. Chargaff’s rule states that ?
A. A=T, G=C
B. A=G, T=C
C. A=C, G=T
D. Any combination possible
Answer: A
548. Only vitamin that help in carbon fixation ?
A. Folic acid
B. Pantothenic acid
C. Niacin
D. Thiamine
Answer: A
549. Rate controlling enzyme of fatty acid synthesis
A. Thioesterase
B. Transacetylase
C. Acetyl-CoA carboxylase
D. Ketacyl synthase
Answer: C
550. Primer function is in ?
A. Transcription
B. Translation
C. Initiation of DNA replication
D. Termination of DNA replication
Answer: C
551. Which of the following is a ribozyme?
A. Peptidyl transferase
B. Elongation factor 2
C. Primase
D. RNA polymerase
Answer: A
552. Which RNA is used in RNA splicing ?
A. mRNA
B. Small nuclear RNA
C. Small cytosolic RNA
D. tRNA
Answer: B
553. What is attatched to 3′ end of mRNA after transcription?
A. Poly Atail
B. CCA
C. Intron
D. 7-methylguanosine
Answer: A
554. Primase functions as ?
A. Joining DNA fragments
B. Synthesining small RNA fragments during DNA synthesis
C. Synthesising small RNA fragments during translation
D. Unwinding of DNA
Answer: B
555. What is the function of DNA ligase ?
A. Unwinding (denaturation) of dsDNA to provide an ssDNA template
B. Seals the single strand nick between the nascent chain and Okazaki fragments on lagging strand
C. Initiation of DNA synthesis and elongation
D. Initiates synthesis of RNA primers
Answer: B
556. Central Dogma of molecular biology indudes all except ?
A. Transcription
B. Translation
C. RNA replication
D. DNA replication
Answer: C
557. Nucleotide consists of all except ?
A. Sugar
B. Phosphate
C. Fatty acid
D. Base
Answer: C
558. Which of the following is required for fatty acid synthesis ?
A. NADPH
B. NADH
C. FADH
D. None
Answer: A
559. Shine dalgarno sequence is related to ?
A. Transcription
B. Translation
C. DNA replication
D. None
Answer: B
560. Citrate used in fatty acid synthesis uses which enzyme ?
A. Citrate Synthase
B. ATP citrate lyase
C. Aconitase
D. Malic enzyme
Answer: B
561. Which of the following is w-6 fatty acid
A. Cervonic acid
B. Linoleic acid
C. Alpha linolenic acid
D. Elaidic acid
Answer: B
562. Linoleic acid is A. w-3 fatty acid
B. w-6 fatty acid
C. w-9 fatty acid
D. Saturated fatty acid
Answer: B
563. Watson crick model is for which DNA ?
A. B DNA
B. A DNA
C. C DNA
D. Z DNA
Answer: A
564. Lipogenesis occurs in ?
A. Liver
B. Skeletal muscles
C. Myocardium
D. Lungs
Answer: A😀
565. Which of the following is monoenoic acid ?
A. Arachidonic acid
B. Linoleic acid
C. Oleic acid
D. Linolenic acid
Answer: C
566. Which of the following is not involved in synthesis of pyrimidines?
A. Glutamine
B. CO
C. Aspartic acid
D. Glycine
Answer: D
567. Which of the following fatty acid has maximum number of carbon atoms ?
A. Oleic acid
B. Linolenic acid
C. Arachidonic acid
D. Cervonic acid
Answer: D
568. Chylomicron remnants are associated with ?
A. Apo-A
B. Apo-B100
C. Apo-E
D. Apo-C
Answer: C
569. Purine are formed by ?
A. Aspartic acid, glycine, uric acid
B. Aspartate, glycine,
C. Aspartate, glutamate
D. Aspartate, glycine, glutamine
Answer: D
570. Apoprotein – C ?
A. Activates lipoprotein lipase
B. Inactivates lipoprotein lipase
C. Facilitates triglyceride transport
D. All of the above
Answer: D
571. Which is not true of chylomicrons ?
A. Lowest desnsity
B. Max. content is TGs
C. Max. content is cholesterol
D. Largest size
Answer: C
572. Beta-alanine is derived from ?
A. Adenosine
B. Guanosine
C. Thymine
D. Uracil
Answer: D
573. Oxidised LDL is more athreogenic because ?
A. Binds to Apo E
B. Binds to scavenger R
C. Binds to ATP binding
D. Accumulates in macrophages
Answer: D
574. Adenine phosphoribosyl transferase is involved ?
A. De novo purine synthesis
B. Purine degradation
C. Salvage synthesis of purine nucleotides
D. None
Answer: C
575. Hormone sensitive lipase is inhibited by?
A. Thyroid homone
B. Insulin
C. GH
D. ACTH
Answer: B
576. Lecithine hydrolysis yeilds ?
A. Choline
B. Pyruvate
C. Glycine
D. None
Answer: A
577. Which of the following requires vitamin BIZ ?
A. Serine to lysine
B. Homocysteine to methionine
C. Serine to glycine
D. Glutamine to glutamate
Answer: B
578. The pyruvate utilization in tissues is decreased in ?
A. Pernicious anemia
B. Scurvy
C. Beriberi
D. Pellagra
Answer: C
579. Coenzyme form of pyridoxine is ?
A. ADP
B. NAD
C. PLP
D. FAD
Answer: C
580. Omega oxidation of fathy acids occur?
A. ER
B. Mitochondria
C. Cytosol
D. None
Answer: A
581. Most potent anti oxidant ?
A. Vit A
B. Vit K
C. Vit E
D. Vit C
Answer: C
582. What is true regarding medium chain fatty acids ?
A. Don’t require pancreatic lipase
B. Not deposited in adipose tissue
C. Diffuse directly into portal circulation
D. All of the above
Answer: D
583. What will you give to stop chyluria in diet?
A. Small chain FA
B. Medium chain FA
C. Long chain FA
D. Omega 3 unsaturated FA
Answer: B
584. Pantothenic acid is needed for donating the following moiety ?
A. Acetyl (or acyl) CoA
B. Carboxyl
C. Hydroxyl
D. Amino
Answer: A
585. Ascorbic acid is required for synthesis of ?
A. Phenylserine
B. Homoserine
C. Hydroxylysine
D. Selenocysteine
Answer: C
586. In a person fasting overnight with carnitine deficiency, following chemicals increase in quantity in blood ?
A. Glucose
B. Fatty acids
C. Amino acids
D. Ketone bodies
Answer: B
587. Which of the following is a Fat Soluble vitamin ?
A. Thiamine
B. Niacine
C. Vitamin A
D. Ribaflavin
Answer: C
588. Serum appeary milky white in ?
A. Increased LDL
B. Increased HDL
C. Increased VLDL
D. Increased Chylomicrons
Answer: D
589. Overnight fasting what occurs ?
A. Glucose decreases
B. FFA increases
C. Increased gluconeogenesis
D. Increased beta-hydroxybutyrate
Answer: C
590. Adipose tissue fat metabolism is done by ?
A. Lipoprotein lipase
B. Hormone sensitive lipase
C. Acid lipase
D. Acid maltase
Answer: B
591. RQ is least in ?
A. Brain
B. RBC
C. Adipose
D. Heart
Answer: D
592. Enzyme deficient in Type I Hyperlipidemia is ?
A. HMG CoA reductase
B. Lipoprotein lipase
C. Cholesterol acyl transferase
D. Peroxidase
Answer: B
593. Apo-E dificiency is seen in
A. Tpye I hypolipoproteinemia
B. Tpye II hypolipoproteinemia
C. Tpye III hypolipoproteinemia
D. Tpye IV hypolipoproteinemia
Answer: C
594. Substance with highest thermogenic effect ?
A. Fat
B. Proteins
C. Carbohydrate
D. All are the same
Answer: B
595. Anaplerotic reaction is catalyzed by ?
A. Pyruvate carboxylse
B. Enolase
C. Pyruvate kinase
D. G6PD
Answer: A
596. Which of the following is not affected in Abetalipoproteinemia ?
A. LDL
B. VLDL
C. HDL
D. IDL
Answer: C
597. In Zellweger syndrome,there is ?
A. Accumulation of long fatty acids
B. Accumulation of short chain fatty acids
C. Accumulation of very long chain fatty acids
D. Accumulation of medium chain fatty acids
Answer: C
598. Autooxidation is seen in ?
A. Cholesterol
B. Arachidonic acid
C. Stearic acid
D. Palmitic acid
Answer: B
599. Which of the following is a lipotropic factor : ?
A. Sphingomyelin
B. Histidine
C. Methionine
D. Bilirubin
Answer: C
600. Lipogenesis is stimulated by ?
A. Insulin
B. Glucagon
C. Epinephrine
D. Corticosteroids
Answer: A
601. Indole ring is present in ?
A. Tryptophan
B. Tyrosine
C. Phenylalanine
D. Threonine
Answer: A
602. Taurine is biosynthesized by ?
A. Arginine
B. Leucine
C. Valine
D. Cysteine
Answer: D
603. Which of the following is true ?
A. Glucokinase has high affinity for glucose
B. Hexokinase has low affinity for glucose
C. Glucokinase has low affinity for glucose
D. Hexokinase is induced by insulin
Answer: C
604. Methylmalonyl aciduria is seen in deficiency of ?
A. Vit B12
B. Vit B6
C. Vit C
D. Folic acid
Answer: A
605. Carnitine is synthesised from A. Lysine
B. Agrinine
C. Histidine
D. Choline
Answer: A
606. Chemiosmotic coupling of oxidation phosphorylation is related to ?
A. Formation of ATP at substrate level
B. ATP generation of pumping of proton
C. ATP generation of pumping of neutron
D. ATP formation by transport of 0,
Answer: B
607. Which one of the following statements concerning gluconeogenesis is correct ?
A. It occurs in muscle
B. It is stimulated by fructose 2, 6-bisphosphate
C. It is inhibited by elevated levels of acetyl CoA
D. It is important in maintaining blood glucose during the normal
overnight fast.
Answer: D
608. Fatty acid metabolism gives ?
A. Acetyl CoA
B. Malonyl CoA
C. Ketone bodies
D. Cholesterol
Answer: A
609. Tyrsoine is the precursor of all except ?
A. Thyroxine
B. Melanin
C. Dopmine
D. Nicotinic acid
Answer: D
610. Organ which can utilize glucose, FA and ketone bodies is
A. Liver
B. Brain
C. Skeletal muscle
D. RBC
Answer: C
611. Which of the following enzyme is not used by liver in urea cycle ?
A. CPS-I
B. CPS-II
C. Arginase
D. Arginosuccinate
Answer: B
612. HMG-CoA in liver mitochondria is inhibited by ?
A. Insulin
B. Glucagon
C. Glucocorticoid
D. Epinephrine
Answer: A
613. Amino acid used by liver in urea cycle ?
A. Glutamine
B. Glutamate
C. Aspartate
D. Fumarate
Answer: C
614. Hydrolysis occurs at which step of urea cycle ?
A. Cleavage of arginine
B. Formation of Arginosuccinate
C. Formation of citrulline
D. Formation of ornithine
Answer: A
615. Why citric acid cycle called amphibolic pathway ?
A. Both exergonic and endergonic reactions takes place
B. Metabolites are utilized in other pathways
C. It can proceed both in forward and backward direction
D. Same enzymes can be used in reverse directions
Answer: A
616. Urea & Kreb’s cycle are linked at ?
A. Arginine
B. Ornithine
C. Oxaloacetate
D. Fumarate
Answer: D
617. Which of the following metabolites is involved in glycogenolysis, glycolysis and gluconeogenesis ?
A. Galactose-l-phosphate
B. Glucose-6-phosphate
C. Uridine diphosphoglucose
D. Fructose-6-phosphate
Answer: B
618. Which will activate carbomoyl phosphate synthase I?
A. Alanine
B. N-acetyl glutamate
C. Ornithine
D. None
Answer: B
619. Enzyme deficient in Isovaleric acidemia
A. Isovaleryl CoA dehydrogenase
B. Phenylalanine hydroxylase
C. Arginase
D. None
Answer: A
620. Transamination of Alanine results in formation of ?
A. Oxaloacetate
B. Pyruvate
C. Aspartate
D. Arginine
Answer: B
621. True about alkaptonuria ?
A. Deficiency of Tyrosinase
B. Urine is black
C. Banedict test is not useful
D. Fecl, test gives red colour
Answer: B
622. Cofactors for glutamate dehydrogenase?
A. NAD+
B. FAD
C. FMN
D. FADH2
Answer: A
623. Ochronosis is due to accumulation of ?
A. Homogentisic acid
B. Phenylpyruvate
C. Xanthurenate
D. Glyoxylate
Answer: A
624. Neonatal tyrosenemia is due to deficiency of ?
A. Tyrosinase
B. Fumarylacetoacetate hyroxyase
C. Hydroxyphenyl pyruvate hydroxylase
D. Tyrosine transminase
Answer: C
625. Separation of proteins by their mass ?
A. Electrophoresis
B. Salting out
C. SDS-PAGE
D. Ion exchange chromatography
Answer: C
626. About Denaturation of protein, which is true ?
A. Biological property persists
B. Primary structure lost
C. Always irreversible
D. Mostly renders protein insoluble
Answer: D
627. Two same charged proteins can be separated by all except
A. Agarose
B. DEAE Cellulose
C. Sephadex
D. None of these
Answer: B
628. In glycolysis, inorganic phosphate is used reaction, catalysed by ?
A. Enolase
B. Pyruvate kinase
C. Glyceraldehyde-3-p dehydrogenase
D. Aldolase
Answer: C
629. 250 nm light is absorbed by ?
A. Arginine
B. Alanine
C. Tyrosine
D. Histidine
Answer: C
630. Tyrosinosis is caused due to deficiency of which enzyme?
A. Fumarylacetoacetate hydrolase.
B. p-hydroxy phenylpyruvate dehydrogenase.
C. Tyrosine transaminase.
D. Tyrosine ligase.
Answer: A
631. Lesch–Nyhan syndrome is caused by deficiency of which enzyme?
A. Orotate Phosphoribosyltransferase
B. Uracil phosphoribosyltransferase
C. Quinolinate Phosphoribosyltransferase
D. Hypoxanthine-guanine phosphoribosyltransferase (HGPRT)
Answer: D
632. Fish odor syndrome is caused by deficiency of which enzyme?
A. Fumarylacetoacetate hydrolase
B. Methane monooxygenase
C. Monooxygenase 3 (FMO3)
D. D-amino acid oxidase
Answer: C
633. Galactosemia is due to deficiency of which enzymes
A. Galactose-1-phosphate uridyltransferase
B. HGPRT
C. Galactokinase
D. Epimerase
Answer: A
634. Which of the following is most abundant end product of fatty acid synthesis
A. Oleic acid
B. Palmitic acid
C. Arachidonic acid
D. Glucose
Answer: B
635. About DNA polymerase I which one is correct?
A. Not required in bacteria
B. Repair any damage with DNA
C. Involved in okazaki fragment
D. Participate in DNA replication
Answer: A
636. What does chaperones assist in?
A. Protein Cleavage
B. Protein Folding
C. Protein Degradation
D. Protein Modification
Answer: B
637. Fishy odour occurs due to deficiency of this vitamin from diet
A. Biotin
B. Thiamine
C. Riboflavin
D. Vit. A
Answer: C
638. VMA is excreted in urine in which condition
A. Alkaptonuria
B. Phenylketonuria
C. Pheochromocytoma
D. Diabetic ketoacidosis
Answer: C
639. In Cystinuria all of the following aminoacids reabsorption defect is present, except
A. Lysine
B. Citrulline
C. Arginine
D. Ornithine
Answer: B
640. Fibrinopeptide A and fibrinopeptide B are acidic due to the presence of which amino acids in its structure
A. Serine and threonine
B. Glutamate and aspartate
C. Histidine and lysine
D. Glutamine and valine
Answer: B
641. HIAA in urine present in?
A. Alkaptonuria
B. Albinism
C. Carcinoid
D. Phenylketonuria
Answer: C
642. Nitric oxide acts by increasing ?
A. BRCA 1
B. BRCA 2
C. Interleukin
D. cGMP
Answer: D
643. Phenylketonuria is due to deficiency of:
A. Phenylalanine
B. Phenylalanine hydroxylase ( PAH)
C. Phenylene
D. All of these
Answer: B
644. By which method foreign DNA is introduced into a cell by a virus or viral vector?
A. Transduction
B. Transcription
C. Lysogenic conversion
D. Transformation
Answer: A
645. Which one of the following shows allosteric inhibition?
A. Malonic acid & succinate
B. 2,3 BPG
C. Amino acid alanine & pyruvate kinase
D. Citrate
Answer: B
646. A 4-year-old boy of a first-degree consanguineous couple was noted by the parents to have darkening of the urine to an almost black color when it was left standing. He has a normal sibling, and there are no other medical problems. Growth and development to date are normal. Which of the following is most likely to be elevated in this patient?
A. Methylmalonate
B. Homogentisate
C. Phenylpyruvate
D. a-Ketoisovalerate
Answer: B
647. Which of the following is true about different structures of protein?
A. Secondary structure is the three-dimensional structure of protein
B. Secondary structure is stabilized by disulfide bonds
C. Primary, secondary and tertiary structures destroyed during denaturation
D. Secondary and tertiary structure depends on the sequence of amino acids
Answer: D
648. The insulin glucagon ratio decreased. The enzyme is active at this time?
A. Glucokinase
B. Hexokinase
C. Phosphofructokinase
D. Glucose 6 phosphatase
Answer: D
649. Ochronosis is due to the accumulation of?
A. Homogentisic acid
B. Homogentisic acid
C. Xanthurenate
D. Glyoxylate
Answer: A
650. Bilirubin in serum can be measured by
A. Van den Bergh reaction
B. Ehrlich’s Reaction
C. Schlesinger’s Reaction
D. Fouchet’s Reaction
Answer: A
651. If a sample of DNA if adenine is 28% what will be the amount of Cytosine present
A. 23%
B. 25%
C. 46%
D. 22%
Answer: D
652. Which of the following vitamin at higher doses causes cystoid macular edema
A. Vit A
B. Vit D
C. Vit E
D. Niacin
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Answer: D
653. True statement regarding Huntington’s chorea is
A. There is a loss of function type of mutation
B. It is an autosomal recessive
C. It is a trinucleotide repeat expansion type of disorder
D. Increased number of CAA repeats
Answer: C
654. Addition of which Amino Acid will increase UV absorption
A. Tryptophan
B. Leucine
C. Proline
D. Arginine
Answer: A
655. V- Richest source of vitamin B12 ?
A. Meat
B. Green leafy vegetables
C. Corn oil
D. Sunflower oil
Answer: A
656. Which amino acid is used to synthesize Nitric oxide?
A. Glycine
B. Arginine
C. Tyrosine
D. Threonine
Answer: B
657. True About Noncompetitive antagonist
A. Km remains same, Vmax decreases
B. Km remains same, Vmax decreases
C. Km decreases, Vmax increases
D. Km increases, Vmax increases
Answer: A
658. Werner syndrome associated with premature aging is caused due to a defect in which of the following?
A. Telomerase
B. Caspase
C. DNA topoisomerase
D. DNA helicase
Answer: D
659. Which of the following dietary fiber is insoluble in water?
A. Pectin
B. Lignin
C. Hemicellulose
D. Cellulose
Answer: B
660. According to NCEP-ATP III, which among the following have not been included in metabolic syndrome?
A. High LDL
B. Hypertriglyceridemia
C. Central Obesity
D. Hypertension
Answer: A
661. Which of the following is the basis for the intestine-specific expression of apoprotein B-48?
A. DNA rearrangement and loss
B. DNA rearrangement and loss
C. RNA alternative splicing
D. RNA editing
Answer: D
